Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 23499095Publication DOI: 10.1016/j.carbpol.2012.12.036Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhou Y <zhouyf383
yahoo.cn>; Zhou Y <zhouyf383
nenu.edu.cn>
Institutions: School of Life Sciences, Northeast Normal University, Changchun , China, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
In previous study, an unbranched (1→6)-β-D-glucan with Mw 2.6kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1→6)-β-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5kDa (BIK2), 288.9kDa (BIK10) and 175.5kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four β-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1→6)-β-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1→6)-β-D-glucan, and the effect of (1→6)-β-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1→6)-β-D-glucan, and indicate (1→6)-β-D-glucans from B. inquinans (Fries) are potential immunostimulating agents.
polysaccharide, Bulgaria inquinans (Fries), lymphocyte proliferation, (1→6)-β-D-glucan
Structure type: homopolymer ; n=9, 2600
Location inside paper: conclusion, BIWP2
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, gel filtration, GC, Smith degradation, HPLC, UV, optical rotation measurement, methylation analysis, phenol-sulfuric acid assay, lymphocyte proliferation assay, monosaccharide analysis, dyalysis
Biological activity: comprative analysis of BIK2, BIK10, BIK30 и BIWP2 revealed molecular weight and the branching ratio influence on immonological activities; the (1→6)-β-D-glucan with higher of branching ration and molecular weight shows higher effect on lymphocytes proliferation; The molecular weight and the branching ratio influence the binding capacity between (1→6)-β-D-glucans and β-glucan receptor, and molecular weight is more effective than branching ratio
Related record ID(s): 44873, 44874, 44875
NCBI Taxonomy refs (TaxIDs): 73172Reference(s) to other database(s): GTC:G26777BZ
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There is only one chemically distinct structure:
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