Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperPublication DOI: 10.1007/s00217-013-2016-8Journal NLM ID: 100957634Publisher: Berlin: Springer
Correspondence: anna_bzducha_wrobel
sggw.pl
Institutions: Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW, Warsaw, Poland
The structure of cell wall of yeasts (genus Saccharomyces) is one of the factors that determine their health-promoting properties connected to the presence of b-glucans and mannoprotein. The aim of the study was to determine the influence of glycerol as a carbon source on structural polymers of cell wall (b-glucan and mannoprotein) of probiotic yeasts Saccharomyces cerevisiae var. boulardii and brewer’s yeasts S. cerevisiae R9. Significant increase of the percentage of polysaccharide content in the cell wall dry weight of S. cerevisiae R9 brewer’s yeasts was noted (in the range of 10–20 %) after cultivation in medium containing glycerol at a concentration of 2–5 % and pH 4.0. The highest content of carbohydrates in probiotic yeasts’ cell wall (58 %) was observed after cultivation in medium containing 3 % of glycerol and pH 5.0. The cell wall of probiotic yeasts was characterized by higher content of mannoprotein comparing with cell wall preparation of brewer’s yeasts S. cerevisiae R9 composed mainly of b-glucans. After cultivation in mediums with 2 and 3 % of glycerol, the cell of brewer’s yeasts contained the highest amount of b(1,3/1,6)-glucan in dry weight of the cell wall (about 36 %). Glycerol at a concentration of 3 and 5 % also intensified mannoprotein biosynthesis in cell wall of S. cerevisiae R9, approximating their content to those noted in the cells of probiotic yeasts (about 29 % (w/w) of dry weight of the cell wall) after cultivation in a medium of pH 5.0 containing 3 % of glycerol.
cell wall, β-glucan, Saccharomyces cerevisiae, mannoprotein, probiotic yeast
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1, Fraction 6, Fraction 5
Trivial name: pustulan, β-1,6-glucan, β-(1,6)-glucan
Compound class: CPS, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: enzymatic hydrolysis, statistical analysis, hydrolysis, dialysis, autolysis
Enzymes that release or process the structure: Zymolyase 20T
Related record ID(s): 44858, 44927, 44939, 47842, 47843, 48303, 48366, 48412, 48423, 48443, 49291, 50093
NCBI Taxonomy refs (TaxIDs): 4932,
252598Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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