Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 23886038Publication DOI: 10.1186/1756-0500-6-294Journal NLM ID: 101462768Publisher: London: Biomed Central, 2008
Correspondence: s.bates
ex.ac.uk
Institutions: College of Life and Environmental Sciences, University of Exeter, Exeter, UK, School of Medical Sciences, University of Aberdeen, Aberdeen, UK, Department of Medicine, Radboud University Nijmegen Medical Center, Nijmege, The Netherlands, School of Biosciences and Institute of Microbiology & Infection, The University of Birmingham, Edgbaston, UK
The Candida albicans cell wall is the first point of contact with the host, and its outer surface is heavily enriched in mannoproteins modified through the addition of N- and O-mannan. Previous work, using mutants with gross defects in glycosylation, has clearly identified the importance of mannan in the host-pathogen interaction, immune recognition and virulence. Here we report the first analysis of the MNN1 gene family, which contains six members predicted to act as α-1,3 mannosyltransferases in the terminal stages of glycosylation. We generated single null mutants in all members of the C. albicans MNN1 gene family, and disruption of MNN14 led to both in vitro and in vivo defects. Null mutants in other members of the family demonstrated no phenotypic defects, suggesting that these members may display functional redundancy. The mnn14Δ null mutant displayed hypersensitivity to agents associated with cell wall and glycosylation defects, suggesting an altered cell wall structure. However, no gross changes in cell wall composition or N-glycosylation were identified in this mutant, although an extension of phosphomannan chain length was apparent. Although the cell wall defects associated with the mnn14Δ mutant were subtle, this mutant displayed a severe attenuation of virulence in a murine infection model.
virulence, cell wall, glycosylation, Candida albicans, mannoproteins, MNN1
Structure type: structural motif or average structure
Location inside paper: p. 1, column 1, paragraph 1
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Related record ID(s): 44855, 44856, 44877, 44889, 44899, 44913, 44915, 44916, 44918, 44919, 44920, 44921, 44923, 44925, 44940, 44941
NCBI Taxonomy refs (TaxIDs): 5476Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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