Taxonomic group: protista / Evosea
(Phylum: Evosea)
Organ / tissue: Life stage: trophozoiteAssociated disease: intestinal infections due to Entamoeba [ICD11:
1A36.0 
, ICD11:
XN3S1 ];
amoebic liver abscess [ICD11:
1A36.10 , ICD11:
XN3S1 ];
infection due to Entamoeba histolytica [ICD11:
XN82F ]
Publication DOI: 10.1111/pim.12812Journal NLM ID: 7910948Publisher: Oxford: Wiley
Institutions: Facultad de Ciencias Biologicas, Instituto de Biotecnologia. San Nicolas de los Garza, Universidad Autonoma de Nuevo Leon, Nuevo Leon, Mexico maria.floresgz@uanl.edu.mx, floresgms@yahoo.com
The ubiquitin-proteasome system plays a central role performing several functions to maintain parasite homeostasis. We have reported the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin (EhUb). Here we examined the immunogenicity and antigenicity of carbohydrates in EhUbiquitin. Rabbits were immunized with purified EhUbiquitin or purified recombinant rUb expressed by E. coli. Using Western Blot, we explored the immunogenicity and antigenicity of protein portion and carbohydrates moiety. Interestingly, immunized rabbits produced antibodies to both Ub glycoprotein and rUb; but antibodies against carbohydrates were immunodominant, rather than antibodies to the protein moiety of EhUbiquitin. In addition, we observed that antibodies to protein moiety are not conserved in serum unless antigen is continually administrated. Conversely, anti-Ub glycoprotein antibodies are well maintained in circulation. In humans, infection with Entamoeba histolytica induces strong IgG anti-Ub response. The human antibodies recognize both, the protein moieties and the glycosylated structure. Entamoeba histolytica ubiquitin is immunogenic and antigenic. The glycan moieties are immunodominant and induces IgG. These data open the door to use carbohydrates as potential targets for diagnose tests, drugs and vaccine to prevent this parasitic disease.
antigen, polysaccharides, immunogen, glycans, Entamoeba histolytica, N-linked glycosylation, EhUbiquitin, Ubiquitin
Structure type: structural motif or average structure
Location inside paper: p. e12812-2, Hex4GlcNAc2
Compound class: N-glycan
Contained glycoepitopes: IEDB_123886,IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_141793,IEDB_141807,IEDB_143632,IEDB_144983,IEDB_144995,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_164479,IEDB_548907,IEDB_983930,SB_136,SB_196,SB_197,SB_198,SB_33,SB_44,SB_67,SB_72,SB_73,SB_74,SB_85
Methods: PCR, ELISA, Western blotting, genetic methods, immunization
Comments, role: N-glycans in the ubiquitin glycoprotein; structure from ref. 8, Obregón A, et al., 2019, Exp Parasitol., 196, 38-47, DOI:10.1016/j.exppara.2018.11.003.
Related record ID(s): 4497, 4498, 4499, 7297
NCBI Taxonomy refs (TaxIDs): 294381
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There is only one chemically distinct structure:
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