Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 1368047Publication DOI: 10.1016/0031-9422(92)80250-iJournal NLM ID: 0151434Publisher: Elsevier
Institutions: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Sendai Aoba-ku, Miyagi, Japan, Department of Radiopharmacy, Tohoku College of Pharmacy, Sendai Aoba-ku, Miyagi, Japan
The structure of the mannan of Candida albicans NIH A-207 strain (serotype A) was investigated by adopting mild acetolysis followed by enzymolysis with an Arthrobacter GJM-1 exo-α-mannosidase. The resultant oligosaccharides, from pentaose to octaose (where manp = D-mannopyranose), were identified as manpβ(1-2)manpα(1-2)manpα(1-2)manpα(1-2)manp, manpβ(1-2)manpβ(1-2)manpα(1-2)manpα(1-2)manpα(1-2)manp, manpβ(1-2)manpβ(1-2)manpβ(1-2)manpα(1-2)manpα(1-2)manpα(1-2)manp and manpβ(1-2)manpβ(1-2)manpβ(1-2)manpβ(1-2)manpα(1-2)manpα(1-2)manpα(1-2)manp, respectively. Analyses of α-linked oligosaccharides obtained by acetolysis under conventional conditions gave the same oligosaccharides, from biose to heptaose, as those obtained from the mannans of C. albicans NIH B-792 (serotype B) and J-1012 (serotype A, formerly serotype C).
mannan, cell wall polysaccharide, Candida albicans, fungus, Manno-oligosaccharides, acetolysis, β-(1-2)-linkage
Structure type: oligomer
Location inside paper: p. 1148, column 1, paragraph 3, table 1, M2-I
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 1H NMR, FAB-MS, acid hydrolysis, enzymatic digestion, acetolysis, hydrolysis, protein detection, phenol-sulfuric acid assay, phosphate measurement, antibody precipitation
Comments, role: product of hydrolysis
Related record ID(s): 44961, 44963, 44964, 44965, 44966, 44968, 44970, 44971, 44972, 44973
NCBI Taxonomy refs (TaxIDs): 5476Reference(s) to other database(s): GTC:G53402KW, GlycomeDB:
278, CCSD:
32606, CBank-STR:2845
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
1H NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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