Taxonomic group: protista / Euglenozoa
(Phylum: Euglenozoa)
Host organism: (mammal)
Organ / tissue: cell surface,
Life stage: epimastigoteAssociated disease: Chagas disease [ICD11:
1F53 
, ICD11:
XN56V ];
infection due to Trypanosoma cruzi [ICD11:
XN56V ]
The structure was elucidated in this paperNCBI PubMed ID: 27556227Publication DOI: 10.1590/0001-3765201620160386Journal NLM ID: 7503280Publisher: Academia Brasileira De Ciencias
Correspondence: luciamp
biof.ufrj.br
Institutions: Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Av. Carlos Chagas Filho, 373, 21941902 Rio de Janeiro, RJ, Brasil, Université des Sciences et Technologies de Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France
One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO4 core, substituted by ethanolamine-phosphate (EtNP) on the third distal mannose from inositol, which may or may not have a terminal β Galf unity. These results confirm the classification of the Silvio X10/1 strain in group T. cruzi I. Again, it is noted that the study of T. cruzi surface glycoconjugates confirm the molecular results and the hypothesis that surface glycoconjugates may be interesting biomarker for the differentiation of trypanosomatid strains.
glycans, Trypanosoma cruzi, glycoinositolphospholipids, mucins, Chagas disease, T.cruzi
Structure type: oligomer
Location inside paper: p. 1525, Fig. 7d
Trivial name: mucin oligosaccharide
Compound class: O-glycan
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_137472,IEDB_141794,IEDB_190606,IEDB_241097,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, GC, MALDI-TOF MS, composition analysis, methanolysis, HPLC, permethylation
Related record ID(s): 4500, 4501, 4503, 4504, 4505, 7298
NCBI Taxonomy refs (TaxIDs): 5693
Show glycosyltransferases
NMR conditions: in D2O at 298 K
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Found 
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