Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: thallus
The structure was elucidated in this paperNCBI PubMed ID: 11150667Publication DOI: 10.1111/j.1574-6968.2001.tb09447.xJournal NLM ID: 7705721Publisher: Blackwell Publishing
Correspondence: iacomini
bio.ufpr.br
Institutions: Departamento de Bioquímica, Universidade Federal do Paraná, Curtiba, Brazil, Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, Brazil
β-D-glucans of the laminaran type were prepared from 15 Cladonia spp., Cladonia bellidiflora, Cladonia boryi, Cladonia clathrata, Cladonia connexa, Cladonia crispatula, Cladonia furcata, Cladonia gracilis, Cladonia ibitipocae, Cladonia imperialis, Cladonia miniata, Cladonia penicillata, Cladonia salmonea, Cladonia signata, Cladonia substellata and Cladonia uncialis. They were extracted with 10% aqueous KOH at 100 degrees C, giving polysaccharides with varying yields and proportions of mannose, galactose and glucose. Their aqueous solutions were freeze-thawed giving precipitates of mixed α-glucan (nigeran) and β-glucans, which were isolated and suspended in aqueous 0.5% KOH at 50 degrees C, which preferentially dissolved the β-glucan. In the case of the C. uncialis product, it was subjected to methylation analysis, which gave rise to 2,4,6-tri-O-methylglucitol acetate only, corresponding to (1→3)-linkages. Its specific rotation (+4 degrees ) and one- and two-dimensional nuclear magnetic resonance (NMR) spectra were consistent with β-linkages. 13C and (1)H-1 signals were observed, respectively, at delta 102.8 (C-1), 86.0 (C-3), 76.2 (C-5), 72.6 (C-2), 68.3 (C-4) and 60.7 (C-6), and 4.55 (H-1), 3.31 (H-2), 3.49 (H-3), 3.27 (H-4), 3.27 (H-5), 3.48 (H-6) and 3.72 (H-6'). Similar (13)C-NMR spectra were obtained from the glucans from the other 14 Cladonia spp. The β-D-glucans of the laminaran type seems to be present in all Cladonia spp. being significant for chemotyping since it was observed in every species studied.
nuclear magnetic resonance, lichenized fungus, β-D-glucan, chemotyping, Laminaran, Cladonia spp.
Structure type: homopolymer
Location inside paper: p.67, fig.1(B-H), table 2
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, GC-MS, acid hydrolysis, paper chromatography, extraction, optical rotation measurement, acetylation, methylation analysis, reduction with NaBH4, precipitation
Comments, role: Trebouxia is the most common symbiote; NMR numeric data were given for Cladonia uncialis
Related record ID(s): 45489
NCBI Taxonomy refs (TaxIDs): 48919,
197136,
184091,
198076,
2028451,
174060,
111668,
2496377,
5199,
198080,
204874,
198082,
196686,
195788,
174080Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.8 72.6 86.0 68.3 76.2 60.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.55 3.31 3.49 3.27 3.27 3.48-3.72
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 102.8/4.55 72.6/3.31 86.0/3.49 68.3/3.27 76.2/3.27 60.7/3.48-3.72
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.55 | 3.31 | 3.49 | 3.27 | 3.27 | 3.48
3.72 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.8 | 72.6 | 86.0 | 68.3 | 76.2 | 60.7 |
|
There is only one chemically distinct structure:
Found 
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