Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 22824506Publication DOI: 10.1016/j.carres.2012.06.016Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: villares.ana
inia.es
Institutions: Centro para la Calidad de los Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Universitario Duques de Soria, Soria, Spain
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation.
polysaccharide, carbohydrate, mushroom, Galactan, glucan, Congo red
Structure type: homopolymer ; 140000
Location inside paper: Table 1, Table 2, Fig.3, PH
Trivial name: α-(1,4)-glucan
Compound class: O-polysaccharide, glucan
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, GC, HPLC, enzymatic digestion, extraction, acetylation, methylation analysis, reduction, precipitation, spectrophotometry, Congo Red assay, centrifugation
Related record ID(s): 45691, 45693
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
NMR conditions: in D2O / (C) at 298(H) K
[as TSV]
13C NMR data:
missing...
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.359 3.386 3.928 3.615 3.839 3.812
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.359 | 3.386 | 3.928 | 3.615 | 3.839 | 3.812 |
|
There is only one chemically distinct structure:
Found 
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