Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 22129838Publication DOI: 10.1016/j.carres.2011.10.043Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, India, Division of Plant Biology, Bose Institute, Centenary Building, Kolkata, India
Two different glucans (water-soluble PS-I, water-insoluble PS-II) were isolated from the alkaline extract of the fruit bodies of hybrid mushroom. PS-I was found to consist of only (1→6)-linked β-D-glucopyranose. PS-II was composed of terminal, (1→3,4)-linked, and (1→3)-linked β-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:1. PS-I showed macrophages, splenocytes, and thymocytes activation as well as antioxidant property. On the basis of sugar analysis, methylation analysis, and NMR studies (1H, 13C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of these glucans were established.
polysaccharide, NMR spectroscopy, immunostimulation, antioxidant property, Hybrid mushroom
Structure type: homopolymer ; 220000
Location inside paper: abstract, PS-I, Table 1, p.109, structure
Trivial name: β-(1,6)-glucan
Compound class: O-polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, GLC-MS, NMR-2D, acid hydrolysis, GLC, Smith degradation, paper chromatography, HPLC, GPC, extraction, optical rotation measurement, acetylation, reduction, determination of NO production, precipitation, phenol-sulfuric acid assay, radical scavenging assay, Gerwig method, DEPT, centrifugation, splenocyte proliferation assay, thymocyte proliferation assay
Biological activity: A maximum of 66.5% DPPH radical scavenging activity was observed at 8 mg/mL of PS-I. The EC50 value of the PS-I for DPPH radicals was 2.5 mg/mL. PS-I was found to stimulate splenocytes and thymocytes.
Comments, role: organism: backcross hybrid of hybrid PfloVv12 (Pleurotus sp. Florida * Volvariella volvacea) and Volvariella volvacea
Related record ID(s): 45738
NCBI Taxonomy refs (TaxIDs): 188765,
36659Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.4 73.5 76.0 70.0 75.4 69.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.54 3.34 3.64 3.50 3.52 3.87-4.24
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.4/4.54 73.5/3.34 76.0/3.64 70.0/3.50 75.4/3.52 69.3/3.87-4.24
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.54 | 3.34 | 3.64 | 3.50 | 3.52 | 3.87
4.24 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.4 | 73.5 | 76.0 | 70.0 | 75.4 | 69.3 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Pleurotus sp. Florida x Volvariella volvacea)