Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelium
NCBI PubMed ID: 22399240Publication DOI: 10.1007/s10295-012-1112-2Journal NLM ID: 9705544Correspondence: Túrmina JA <janainaangela
hotmail.com>; Alves da Cunha MA <mcunha
utfpr.edu.br>; Dekker RF <rdekker
lakeheadu.ca>; Barbosa AM <anelibarbosa
gmail.com>
Institutions: Department of Chemistry, Federal Technological University of Parana, Pato Branco, Brazil, Biorefining Research Institute, Lakehead University, Thunder Bay, Canada, Department of Pharmacy, State University of West Paraná, Cascavel, Brazil, Department of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil, Northern Ontario School of Medicine, Lakehead University, Thunder Bay, Canada
Lasiodiplodan, an exopolysaccharide of the (1→6)-β-D-glucan type, is produced by Lasiodiplodia theobromae MMPI when grown under submerged culture on glucose. The objective of this study was to evaluate lasiodiplodan production by examining the effects of carbon (glucose, fructose, maltose, sucrose) and nitrogen sources (KNO3, (NH4)2SO4, urea, yeast extract, peptone), its production in shake flasks compared to a stirred-tank bioreactor, and to study the rheology of lasiodiplodan, and lasiodiplodan's anti-proliferative effect on breast cancer MCF-7 cells. Although glucose (2.05 ± 0.05 g/L), maltose (2.08 ± 0.04 g/L) and yeast extract (2.46 ± 0.06 g/L) produced the highest amounts of lasiodiplodan, urea as N source resulted in more lasiodiplodan per unit biomass than yeast extract (0.74 ± 0.006 vs. 0.22 ± 0.008 g/g). A comparison of the fermentative parameters of L. theobromae MMPI in shake flasks and a stirred-tank bioreactor at 120 h on glucose as carbon source showed maximum lasiodiplodan production in agitated flasks (7.01 ± 0.07 g/L) with a specific yield of 0.25 ± 0.57 g/g and a volumetric productivity of 0.06 ± 0.001 g/(L·h). A factorial 2^2 statistical design developed to evaluate the effect of glucose concentration (20-60 g/L) and impeller speed (100-200 rpm) on lasiodiplodan production in the bioreactor showed the highest production (6.32 g/L) at 72 h. Lasiodiplodan presented pseudoplastic behaviour, and the apparent viscosity increased at 60°C in the presence of CaCl2. Anti-proliferative activity of lasiodiplodan was demonstrated in MCF-7 cells, which was time- and dose-dependent with an IC(50) of 100 μg lasiodiplodan/mL.
Lasiodiplodia theobromae, lasiodiplodan, (1→6)-β-D-glucan, carbon and nitrogen nutrients, anti-proliferative activity, breast cancer cells (MCF-7)
Structure type: homopolymer
Location inside paper: p.1182, right column, paragraph 2
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: IR, acid hydrolysis, HPAEC-PAD, colorimetry, extraction, cell growth, precipitation, centrifugation, MTT
Biological activity: Lasiodiplodan showed anti-proliferative activity in breast cancer (MCF-7) cells.
Related record ID(s): 45912
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
There is only one chemically distinct structure:
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