Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 25256522Publication DOI: 10.1016/j.carbpol.2014.07.057Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Iacomini M <iacomini
ufpr.br>
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil, Departamento de Farmacologia, Universidade Federal do Paraná, Curitiba, PR, Brazil, Universidade da Região de Joinville, Joinville, Brazil
Glucans comprise an important class of polysaccharides present in basidiomycetes with potential biological activities. A (1→3)-β-D-glucan was isolated from Pleurotus sajor-caju via extraction with hot water followed by fractionation by freeze-thawing and finally by dimethyl sulfoxide extraction. The purified polysaccharide showed a 13C-NMR spectrum with six signals consisting of a linear glucan with a β-anomeric signal at 102.8 ppm and a signal at 86.1 ppm relative to O-3 substitution. The other signals at 76.2, 72.9, 68.3, and 60.8 ppm were attributed to C5, C2, C4, and C6, respectively. This structure was confirmed by methylation analysis, and HSQC studies. The β-D-glucan from P. sajor-caju presented an immunomodulatory activity on THP-1 macrophages, inhibited the inflammatory phase of nociception induced by formalin in mice, and reduced the number of total leukocytes and myeloperoxidase levels induced by LPS. Taken together, these results demonstrate that this β-D-glucan exhibits a significant anti-inflammatory activity.
β-D-glucan, fruiting bodies, Pleurotus sajor-caju, Edible mushroom, anti-inflammatory activity
Structure type: homopolymer
Location inside paper: G-PHW, fig.2 (C)
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, HPSEC, extraction, methylation analysis, NaBH4 reduction, biological assay, macrophage activity assay
Biological activity: inhibited TNF-α: 61.8±5.74% (50 mcg/ml, 3 h), 77.5±0.99% (250 mcg/ml, 6 h); inhibited at 250 mcg/ml 37.0±0.67% IL-1β mRNA and 63.6±2.76% COX-2 mRNA; inhibited the nociception of formalin-induced inflammatory pain (second phase)with IC50 2.09 mg/kg and inhibited 98±2% at a dose 10 mg/kg
Comments, role: crude glucan Mw=60000 g/mol
Related record ID(s): 41575, 43791, 46569
NCBI Taxonomy refs (TaxIDs): 50053Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.8 72.9 86.1 68.3 76.2 60.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.41 3.18 3.37 3.12 3.13 3.34-3.59
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 102.8/4.41 72.9/3.18 86.1/3.37 68.3/3.12 76.2/3.13 60.8/3.34-3.59
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.41 | 3.18 | 3.37 | 3.12 | 3.13 | 3.34
3.59 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.8 | 72.9 | 86.1 | 68.3 | 76.2 | 60.8 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Lentinus sajor-caju CCB019)