β-D-glucans are polymers of d-glucose monomers found in the cell walls of many bacteria, plants, fungi and yeasts. A variety of β-D-glucans differing in structures have been isolated from various sources and their biological activity to be regulated by various structural factors, such as the primary structure, molecular weight, solubility, and conformation. This study investigated the effect of extraction time and temperature on the yield of β-D-glucan produced by Rhodotorula mucilaginosa. A statistical Doehlert design was applied to determine the important effects and interactions of these independent variables on the yield of β-D-glucan, the dependent variable. Significant models were obtained. The best yield was of 25% obtained after 128 min of extraction in a temperature of 72 C. The polysaccharides were characterized as (1→3)-β-D-glucan by methods spectroscopic (FT-IR, 1HNMR and 13CNMR). In addition, the antinociceptive effect was evaluated using different experimental tests (acetic acid-induced writhing test, formalin test and tail immersion test). The (1→3)-β-D-glucan showed a potent peripheral antinociceptive effect, possibly by the inhibition of inflammatory mediators.

optimization, response surface methodology, β-D-glucan, Rhodotorula mucilaginosa, Antinociceptive

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
	bDGlcp	105	74.0	86.1	72.0	76.4	61.5


1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@@H]1[C@@H](O)[C@H](*)O[C@H](CO)[C@H]1O

162.141 g/mol (C₆H₁₀R₂O₅, R = next and previous repeats, not counted in mol weight)