Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: thallus
The structure was elucidated in this paperPublication DOI: 10.1016/S0144-8617(99)00048-XJournal NLM ID: 8307156Publisher: Elsevier
Correspondence: iacomini
bio.ufpr.br
Institutions: Departamento de Bioquı́mica, Universidade Federal do Paraná, Curitiba, Brazil, Departamento de Morfologia e Fisiologia Animal, UFRPE, Recife, Brazil
Several structurally different glucans were characterized as components of Ramalina celastri. Aqueous KOH extraction of the lichen at 100°C, followed by dialysis provided amylose (0.02%), identified and quantified by its blue coloration with iodine. The extract was frozen and thawed and the resulting precipitate (2% yield) shown to be a mixture of two insoluble D-glucans, with (1→3)- and (1→3),(1→4)-linkages, respectively, as shown by 13C NMR spectroscopy. On treatment with 0.5% aqueous NaOH at 50°C, the material which remained insoluble was a linear β-glucan with regularly distributed (1→3)- and (1→4)-linkages in a 1:1 molar ratio (nigeran, 1.2% yield), whereas that which solubilised was a linear β-glucan with (1→3)-linkages (laminaran, 0.8% yield). The mother liquor of the KOH extraction was treated with Fehling solution to give a precipitate and the supernatant contained an α-D-glucan (28% yield) with (1→3)- and (1→4)- linkages in a molar ratio of 3:1, and which were distributed irregularly. The structures of these two (1→3),(1→4)-linked β-glucans were characterized by methylation, controlled Smith degradation and 13C and 1H NMR spectroscopic analyses.
β-D-glucan, α-D-Glucans, amylose, Ramalina celastri
Structure type: homopolymer
Location inside paper: fig.3 (1)
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: CPS, EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, periodate oxidation, NMR-2D, GC-MS, GC, Smith degradation, paper chromatography, HPSEC, viscosity measurement, extraction, methylation analysis, NaBH4 reduction, CC, dialysis, iodine reacion
Comments, role: symbiotic organism
Related record ID(s): 47132, 47133, 47134, 47136, 47137, 47138
NCBI Taxonomy refs (TaxIDs): 859457Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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