Taxonomic group: fungi, fungi, plant / Ascomycota, Ascomycota, Streptophyta
(Phylum: Ascomycota, Ascomycota, Streptophyta)
Host organism: Hordeum vulgare; Hordeum vulgare
Organ / tissue: grainAssociated disease: Fusarium head blight
NCBI PubMed ID: 25952699Publication DOI: 10.1080/19440049.2015.1048750Journal NLM ID: 101485040Publisher: Informa Healthcare
Correspondence: veronica.lattanzio
ispa.cnr.it
Institutions: National Research Council of Italy (CNR), Institute of Sciences of Food Production (ISPA), Bari, Italy, Council for Agricultural Research and Economics, Genomics Research Centre, CRA-GPG, Fiorenzuola d’Arda, Italy, United States Department of Agriculture, Bacterial Foodborne Pathogens & Mycology Research Unit, National Center for Utilization Research Laboratory, Peoria, IL, USA
This paper reports a new method for the determination of T-2 and HT-2 toxins and their glucosylated derivatives in cereals, and some survey data aimed at obtaining more comprehensive information on the co-occurrence of T-2 and HT-2 toxins and their glucosylated derivatives in naturally contaminated cereal samples. For these purposes, barley samples originating from a Northern Italian area were analysed by LC-HRMS for the presence of T-2, HT-2 and relevant glucosyl derivatives. Quantitative analysis of T-2 and HT-2 glucosides was performed for the first time using a recently made available standard of T-2 glucoside. The glucosyl derivative of HT-2 was detected at levels up to 163 µg/kg in 17 of the 18 analysed unprocessed barley grains, whereas the monoglucosyl derivative of T-2 toxin was detected in only a few samples and at low µg/kg levels. The ratio between glucosylated toxins (sum of T-2 and HT-2 glucosides) and native toxins (sum of T-2 and HT-2) ranged from 2% to 283%. Moreover, taking advantage of the possibility of retrospective analysis of full-scan HRMS chromatograms, samples were also screened for the presence of other type-A trichothecenes, namely neosolaniol, diacetoxyscirpenol and their monoglucosyl derivatives, which were detected at trace levels. A subset of nine different samples was subjected to micro-maltation in order to carry out a preliminary investigation on the fate of T-2, HT-2 and relevant glucosides along the malting process. Mycotoxin reduction from cleaned barley to malt was observed at rates ranging from 4% to 87%
HT-2 toxin, T-2 toxin, barley, neosolaniol, diacetoxyscirpenol, trichothecene glucosides, modified mycotoxins, high resolution mass spectrometry, malt
Structure type: monomer ; 562.2500 [M+NH4]+
Location inside paper: Fig. 2, NEO-glucoside
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: enzymatic digestion, extraction, LC-ESI-MS, LC-HRMS
Related record ID(s): 47918, 47919, 47921
NCBI Taxonomy refs (TaxIDs): 5506,
378046,
4513
Show glycosyltransferases
There is only one chemically distinct structure:
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