Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii AB5075 and studied by 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: →3)-β-D-ManpNAcA-(1→4)-β-D-ManpNAcA-(1→3)-α-D-QuipNAc4NR-(1→ where R indicates (S)-3-hydroxybutanoyl or acetyl in the ratio ∼ 2.5:1. The genes in the polysaccharide biosynthesis locus designated KL25 are appropriate to the established CPS structure.

Acinetobacter baumannii, capsular polysaccharide structure, polysaccharide gene locus

NCBI PubMed ID: 25816997
Publication DOI: 10.1016/j.carres.2015.02.011
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: yknirelgmail.com
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, ND. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, NMR-1D, GPC, bioinformatic analysis
 

• Compound ID: 12013
  

  /Variants 0/-+ | -3)-b-D-ManpNAcA-(1-4)-b-D-ManpNAcA-(1-3)-a-D-QuipNAc4N-(1- /Variants 0/ is: 71%S-3HOBut-(1-4)- OR (exclusively) 29%Ac-4)-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  
   
  
  Acinetobacter baumannii AB5075 (MRSN959)
  CSDB ID 30670 (all data & tools)