Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
NCBI PubMed ID: 25187160Publication DOI: 10.1093/glycob/cwu091Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: nshibata
tohoku-pharm.ac.jp
Institutions: Department of Infection and Host Defense, Tohoku Pharmaceutical University, Sendai, Japan, The Sendai Open Hospital, Sendai, Japan
Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies are present in these results. In this study, to clarify the relationship between the growth conditions and structure of the galactomannans, we cultured A. fumigatus using two distinct yeast/fungal cultivation media, i.e. the yeast extract-peptone-dextrose (YPD) medium and yeast nitrogen base (YNB) medium. Galactomannans prepared from the resulting culture supernatants were structurally characterized by 1H and 13C nuclear magnetic resonance, methylation analysis, acetolysis and α-mannosidase degradation. These assays revealed that the galactomannan from the YPD cultivation had short β-1,5-linked galactofuranose (Galf) oligosaccharide chains in both the O- and N-linked carbohydrate moieties, while the galactomannan from the YNB cultivation incorporated long Galf oligosaccharide chains. The galactomannans derived from the two culture conditions significantly differed in reactivity based on the EIA diagnostic system. We also demonstrated the presence of a novel Galf-containing branched oligosaccharide in the O-linked moiety
NMR, antigen, oligosaccharide, Galactomannan, Aspergillus
Structure type: oligomer
Location inside paper: Table II
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, enzyme immunoassay, acid hydrolysis, alkaline hydrolysis, enzymatic digestion, acetylation, acetolysis, methylation analysis, reduction, CC, gel permeation chromatography, cell growth, dialysis, enzymatic assay, precipitation, evaporation, DEPT
Enzymes that release or process the structure: α-mannosidase (EC 3.2.1.24)
Related record ID(s): 48307, 48308, 48309, 48310, 48311, 48312, 48313, 48314, 48315, 48316, 48317, 48318, 48319, 48320, 48321, 48322, 48323, 48324, 48325
NCBI Taxonomy refs (TaxIDs): 746128Reference(s) to other database(s): GTC:G53402KW, GlycomeDB:
278, CCSD:
32606, CBank-STR:2845
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error