Taxonomic group: protista / Euglenozoa
(Phylum: Euglenozoa)
Host organism: Homo sapiens
Organ / tissue: cell surface,
Life stage: epimastigoteAssociated disease: Chagas disease [ICD11:
1F53 
, ICD11:
XN56V ];
infection due to Trypanosoma cruzi [ICD11:
XN56V ]
The structure was elucidated in this paperNCBI PubMed ID: 23578542Publication DOI: 10.1016/j.carres.2013.03.010Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: H. Verli <hverli
cbiot.ufrgs.br>
Institutions: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91500-970, RS, Brazil
Trypanosoma cruzi is a protozoan, responsible for Chagas disease, that parasites triatomines and some vertebrates, mainly Homo sapiens. In 2010, nearly 10 million people in whole world, most from Latin America, had Chagas disease, which is an illness of high morbidity, low mortality, and serious problems of quality of life. The available treatment has high toxicity and low efficacy at chronic phase. Some of the protozoan antigenic or virulence factors include complex carbohydrate structures that, due to their uniqueness, may constitute potential selective targets for the development of new treatments. One example of such structures is NETNES, a low abundance T. cruzi glycopeptide, comprising 13 amino acid residues, one or two N-glycosylation chains, a GPI anchor and two P-glycosylations. In this context, the current work aims to obtain an atomic model for NETNES, including its glycan chains and membrane attachment, in order to contribute in the characterization of its structure and dynamics. Based on POPC and GPI models built in agreement with experimental data, our results indicate that, in the first third of the simulation, NETNES peptide is very flexible in solution, bending itself between asparagine residues and lying down on some carbohydrates and membrane, exposing amino acid residues and some other glycans, mainly terminal mannoses, to the extracellular medium, remaining in this position until the end of simulations.
molecular dynamics, glycopeptide, Trypanosoma cruzi, N-glycosylation, GPI anchor, NETNES
Structure type: oligomer
Location inside paper: p.33, Fig.6, P-glycosylation of NETNES
Aglycon: Asn
Trivial name: glycan chain of NETNES glycopeptide
Compound class: glycan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141795,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_144996,IEDB_152206,IEDB_164480,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: conformation analysis, MD simulations, topological information
Related record ID(s): 4837, 4839, 4840, 4841, 7274
NCBI Taxonomy refs (TaxIDs): 5693
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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