Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
NCBI PubMed ID: 26340350Publication DOI: 10.1371/journal.pone.0137230Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: takegawa
agr.kyushu-u.ac.jp
Institutions: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Japan, Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
β-D-galactofuranose (Galf) is a component of polysaccharides and glycoconjugates and its transferase has been well analyzed. However, no β-D-galactofuranosidase (Galf-ase) gene has been identified in any organism. To search for a Galf-ase gene we screened soil samples and discovered a strain, identified as a Streptomyces species by the 16S ribosomal RNA gene analysis, that exhibits Galf-ase activity for 4-nitrophenyl β-D-galactofuranoside (pNP-β-D-Galf) in culture supernatants. By draft genome sequencing of the strain, named JHA19, we found four candidate genes encoding Galf-ases. Using recombinant proteins expressed in Escherichia coli, we found that three out of four candidates displayed the activity of not only Galf-ase but also α-L-arabinofuranosidase (Araf-ase), whereas the other one showed only the Galf-ase activity. This novel Galf-specific hydrolase is encoded by ORF1110 and has an optimum pH of 5.5 and a Km of 4.4 mM for the substrate pNP-β-D-Galf. In addition, this enzyme was able to release galactose residue from galactomannan prepared from the filamentous fungus Aspergillus fumigatus, suggesting that natural polysaccharides could be also substrates. By the BLAST search using the amino acid sequence of ORF1110 Galf-ase, we found that there are homolog genes in both prokaryotes and eukaryotes, indicating that Galf-specific Galf-ases widely exist in microorganisms
Streptomyces, genome sequencing, β-D-galactofuranosidase, ORF1110
Structure type: structural motif or average structure ; n=9-10
Location inside paper: Fig. 6, B
Compound class: polysaccharide, galactomannan
Contained glycoepitopes: IEDB_130701,IEDB_136095,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141795,IEDB_141830,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_147453,IEDB_149137,IEDB_152206,IEDB_164480,IEDB_190606,IEDB_76933,IEDB_885812,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: DNA techniques, TLC, ELISA, cell growth, dialysis, evaporation, centrifugation, BLAST
Enzymes that release or process the structure: ORF1110 β-D-galactofuranosidase
NCBI Taxonomy refs (TaxIDs): 746128
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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