Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 26344312Publication DOI: 10.1016/j.carbpol.2015.07.060Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Cordeiro LM <lucimaramcc
ufpr.br>
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil, Biorefining and Biotechnology Consultancy, Londrina, Brazil, Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), Assis, Brazil, Departamento de Química, Universidade Estadual de Londrina, Londrina, Brazil
The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1800000 Da and 7000 Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern
exopolysaccharides, immunomodulatory activity, lasiodiplodan, (1→3)(1→6)-β-glucan, linear (1→6)-β-glucan
Structure type: homopolymer ; 7000-1800000
Location inside paper: Fig. 3
Compound class: EPS, glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, GC-MS, acid hydrolysis, Smith degradation, HPSEC-MALLS, acetylation, methylation analysis, reduction, cell growth, dialysis, cytokine production, cell viability assay, precipitation, macrophage activity assay, DEPT, centrifugation, MTT, ultrafiltration
Biological activity: polysaccharide induces a proinflammatory response pattern, which has been correlated with antitumor action. Polysaccharide displays no toxicity toward U937 macrophage cells or PBMC (peripheral mononuclear blood cells)
Related record ID(s): 7724, 8325, 42140, 43301, 43379, 44888, 44927, 44939, 45578, 47842, 47843, 48303, 48366, 48412, 48422, 48443, 49291, 101372, 124916, 138263, 143680, 143682, 149870
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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