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Taxonomic group: protista / Euglenozoa (Phylum: Euglenozoa)
Host organism: Homo sapiens
Organ / tissue: Life stage: procyclic promastigote
Associated disease: infection due to Leishmania [ICD11: XN8JE

]; visceral leishmaniasis [ICD11: 1F54.0

, ICD11: XN8JE

]

The structure was elucidated in this paper
NCBI PubMed ID: 21118695
Publication DOI: 10.1016/j.ijpara.2010.10.004
Journal NLM ID: 0314024
Publisher: Int J Parasitol
Correspondence: rsoares

cpqrr.fiocruz.br
Institutions: Laboratory of Medical Entomology, Centro de Pesquisas Rene Rachou/FIOCRUZ, Belo Horizonte, MG, Brazil

Interspecies variations in lipophosphoglycan (LPG) have been the focus of intense study over the years due its role in specificity during sand fly-Leishmania interaction. This cell surface glycoconjugate is highly polymorphic among species with variations in sugars that branch off the conserved Gal(β1,4)Man(α1)-PO(4) backbone of repeat units. However, the degree of intraspecies polymorphism in LPG of Leishmania infantum (syn. Leishmania chagasi) is not known. In this study, intraspecific variation in the repeat units of LPG was evaluated in 16 strains of L. infantum from Brazil, France, Algeria and Tunisia. The structural polymorphism in the L. infantum LPG repeat units was relatively slight and consisted of three types: type I does not have side chains; type II has one β-glucose residue that branches off the disaccharide-phosphate repeat units and type III has up to three glucose residues (oligo-glucosylated). The significance of these modifications was investigated during in vivo interaction of L. infantum with Lutzomyia longipalpis, and in vitro interaction of the parasites and respective LPGs with murine macrophages. There were no consequential differences in the parasite densities in sand fly midguts infected with Leishmania strains exhibiting type I, II and III LPGs. However, higher nitric oxide production was observed in macrophages exposed to glucosylated type II LPG.

interaction, leishmania, lipophosphoglycan, Leishmania infantum, Intraspecific variation, Lutzomyia longipalpis

Structure type: oligomer
Location inside paper: table 1, p. 339, fig. 6 (III), LPG - type III
Compound class: LPG
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_135614,IEDB_136044,IEDB_136095,IEDB_136100,IEDB_136906,IEDB_137472,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144996,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153218,IEDB_153543,IEDB_158555,IEDB_164174,IEDB_190606,IEDB_241101,IEDB_433717,IEDB_474450,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: dephosphorylation, FACE, HPLC, immunoblotting, statistical analysis, CE, enzymatic treatment, sand fly infections
Comments, role: ALK = C24 or C26; side chains in type-III LPG repeats, are containing up to three glucose residues, but the precise locations of the Glc side chains in the repeat unit are not known.

Related record ID(s): 4843, 7276
NCBI Taxonomy refs (TaxIDs): 5671
Show glycosyltransferases

There are too many chemically distinct structures (~147456), so only one is shown:

ALK(1-1)[44%bDGlcp(1-2)23%bDGlcp(1-2)%bDGlcp(1-2)[44%bDGlcp(1-2)23%bDGlcp(1-2)%bDGlcp(1-2)[44%bDGlcp(1-2)23%bDGlcp(1-2)%bDGlcp(1-2)bDGalp(1-4)aDManp(1-P-6)]bDGalp(1-4)aDManp(1-P-6)]bDGalp(1-4)aDManp(1-P-6)aDGalp(1-6)aDGalp(1-3)bDGalf(1-3)[aDGlcp(1-P-6)]aDManp(1-3)aDManp(1-4)aDGlcpN(1-6)xLmyoIno?(1-P-3)]x?Gro?

[1*]OCC(O)COP(=O)(O)O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](COP(=O)(O)O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](O[C@@H]4O[C@@H]([C@H](O)CO)[C@H](O[C@H]5O[C@H](CO[C@H]6O[C@H](COP(=O)(O)O[C@H]7O[C@H](CO)[C@@H](O[C@@H]8O[C@H](COP(=O)(O)O[C@H]9O[C@H](CO)[C@@H](O[C@@H]%10O[C@H](COP(=O)(O)O[C@H]%11O[C@H](CO)[C@@H](O[C@@H]%12O[C@H](CO)[C@H](O)[C@H](O)[C@H]%12O[C@@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O[C@@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O[C@@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)[C@H](O)[C@@H]%11O)[C@H](O)[C@H](O)[C@H]%10O[C@@H]%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O[C@@H]%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O[C@@H]%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O)[C@H](O)[C@@H]9O)[C@H](O)[C@H](O)[C@H]8O[C@@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O[C@@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O[C@@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@H](O)[C@@H]7O)[C@H](O)[C@H](O)[C@H]6O)[C@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O)[C@H]1N

SVG MW SMILES 3D mol Ionize

R1 = ALK
Warning = sub-repeat ending at xXP?: unknown unit count was assumed to be 3