Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 8620883Publication DOI: 10.1111/j.1432-1033.1996.0266n.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: School of Chemistry, University of Hull, UK.
A major polysaccharide containing D-galactose, D-glucose and 2-acetamido-2-deoxy-D-galactose was obtained after mild acid hydrolysis of the water-soluble material released by treatment of cell walls from Acinetobacter baumannii strain O11 with hot, aqueous phenol. By means of NMR studies, Smith degradation and N-deacetylation/deamination, the repeating unit of the polymer was identified as a branched pentasaccharide of the structure shown. Also present was a minor polymer containing glucose, 2-acetamido-2-deoxyglucose-and 2-acetamido-2-deoxygalactose, the structure of which was not elucidated. On serological testing, the polymeric material was shown to correspond to the O-antigenic moiety of the parent extract (assumed to be lipopolysaccharide) and circumstantial evidence indicated that O11 specificity was conferred by the major polymer. [formula: see text]
structure, O-antigen, Acinetobacter baumannii, O-specific polysaccharide, lipopolyysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.270, Table 1
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_141584,IEDB_141794,IEDB_142488,IEDB_143260,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_742248,IEDB_885822,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: NMR, Smith degradation, de-N-acetylation/deamination
Comments, role: published polymerization frame was shifted for conformity with other records
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G69238MT, GlycomeDB:
25206
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6,3,2 Ac 175.06-176.06 23.08-23.08
3,6,3,6 bDGlcp 103.80 74.02 76.76 70.52 76.77 61.68
3,6,3 bDGalpN 104.03 53.90 71.36 76.90 73.36-74.62 69.00-69.06
3,6 aDGalp 99.92 68.24 80.32 70.07 71.60 62.04
3 bDGalp 105.16 71.68 73.60 69.46 73.36-74.62 67.36
2 Ac 175.06-176.06 23.08-23.08
aDGalpN 99.80 49.68 78.32 69.00-69.06 71.28 61.68
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6,3,2 Ac - 2.05-2.08
3,6,3,6 bDGlcp 4.42 3.29 3.46 3.43 3.40 3.73-3.90
3,6,3 bDGalpN 4.72 4.02 3.82 4.08 3.90 3.90-4.00
3,6 aDGalp 4.97 3.93 3.89 4.20 3.92 3.76
3 bDGalp 4.58 3.57 3.67 4.03 3.90 3.80
2 Ac - 2.05-2.08
aDGalpN 5.04 4.39 4.13 4.30 4.39 3.72
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6,3,2 Ac 23.08-23.08/2.05-2.08
3,6,3,6 bDGlcp 103.80/4.42 74.02/3.29 76.76/3.46 70.52/3.43 76.77/3.40 61.68/3.73-3.90
3,6,3 bDGalpN 104.03/4.72 53.90/4.02 71.36/3.82 76.90/4.08 73.36-74.62/3.90 69.00-69.06/3.90-4.00
3,6 aDGalp 99.92/4.97 68.24/3.93 80.32/3.89 70.07/4.20 71.60/3.92 62.04/3.76
3 bDGalp 105.16/4.58 71.68/3.57 73.60/3.67 69.46/4.03 73.36-74.62/3.90 67.36/3.80
2 Ac 23.08-23.08/2.05-2.08
aDGalpN 99.80/5.04 49.68/4.39 78.32/4.13 69.00-69.06/4.30 71.28/4.39 61.68/3.72
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6,3,2 | Ac |
| 2.05
2.08 | |
| 3,6,3,6 | bDGlcp | 4.42 | 3.29 | 3.46 | 3.43 | 3.40 | 3.73
3.90 |
| 3,6,3 | bDGalpN | 4.72 | 4.02 | 3.82 | 4.08 | 3.90 | 3.90
4.00 |
| 3,6 | aDGalp | 4.97 | 3.93 | 3.89 | 4.20 | 3.92 | 3.76 |
| 3 | bDGalp | 4.58 | 3.57 | 3.67 | 4.03 | 3.90 | 3.80 |
| 2 | Ac |
| 2.05
2.08 | |
| | aDGalpN | 5.04 | 4.39 | 4.13 | 4.30 | 4.39 | 3.72 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6,3,2 | Ac | 175.06
176.06 | 23.08
23.08 | |
| 3,6,3,6 | bDGlcp | 103.80 | 74.02 | 76.76 | 70.52 | 76.77 | 61.68 |
| 3,6,3 | bDGalpN | 104.03 | 53.90 | 71.36 | 76.90 | 73.36
74.62 | 69.00
69.06 |
| 3,6 | aDGalp | 99.92 | 68.24 | 80.32 | 70.07 | 71.60 | 62.04 |
| 3 | bDGalp | 105.16 | 71.68 | 73.60 | 69.46 | 73.36
74.62 | 67.36 |
| 2 | Ac | 175.06
176.06 | 23.08
23.08 | |
| | aDGalpN | 99.80 | 49.68 | 78.32 | 69.00
69.06 | 71.28 | 61.68 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
