Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 8620877Publication DOI: 10.1111/j.1432-1033.1996.0229n.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: School of Chemistry, University of Hull, England.
polysaccharide containing 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamido-2-deoxy-L-fucose (FucNAc), and 2-acetamido-2-deoxy-D-galacturonic acid (GalNAcA) was isolated from an aqueous phenol extract of lipid-free, isolated cell walls of the reference strain for Acinetobacter baumannii serogroup O5, by mild acid hydrolysis of the extract and chromatography of the water-soluble products on Sephadex G-50. By means of NMR studies, methylation analysis, carboxyl reduction and chemical degradations, the repeating unit of the polymer was identified as a branched tetrasaccharide of the structure shown. The serologically active polymer is believed to correspond to the side chain of the O5 lipopolysaccharide: [table: see text]
structure, polysaccharide, acid, Acinetobacter, Acinetobacter baumannii, O-specific, O-specific polysaccharide, 2-acetamido-2, 6-dideoxy-L-galactose, 2-acetamido-2-deoxy-D-galacturonic acid
Structure type: polymer chemical repeating unit
Location inside paper: abstract, compound 3
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531
Methods: methylation, NMR-2D, partial acid hydrolysis, NMR, carboxyl reduction, Smith degradation
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 506
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G73635WZ, GlycomeDB:
25208
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,2 Ac 173-175 23
3,3,4,2 Ac 173-175 23
3,3,4 aLFucpN 97.36 50.27 68.32 72.00 67.87 16.26
3,3 aDGalpNA 99.07 49.07 77.23 74.48 71.41 173-175
3,2 Ac 173-175 23
3 aLFucpN 98.24 49.22 73.90 72.00 67.53 15.96
2 Ac 173-175 23
bDGlcpN 102.49 56.92 78.63 69.48 76.52 61.77
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,2 Ac - 1.90-2.05
3,3,4,2 Ac - 1.90-2.05
3,3,4 aLFucpN 5.52 4.10 3.89 3.77 3.94 1.19
3,3 aDGalpNA 5.14 4.43 4.18 4.71 4.49 -
3,2 Ac - 1.90-2.05
3 aLFucpN 4.89 4.31 4.01 3.73 4.40 1.15
2 Ac - 1.90-2.05
bDGlcpN 4.68 3.64 3.79 3.49 3.42 3.73-3.93
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,2 Ac 23/1.90-2.05
3,3,4,2 Ac 23/1.90-2.05
3,3,4 aLFucpN 97.36/5.52 50.27/4.10 68.32/3.89 72.00/3.77 67.87/3.94 16.26/1.19
3,3 aDGalpNA 99.07/5.14 49.07/4.43 77.23/4.18 74.48/4.71 71.41/4.49
3,2 Ac 23/1.90-2.05
3 aLFucpN 98.24/4.89 49.22/4.31 73.90/4.01 72.00/3.73 67.53/4.40 15.96/1.15
2 Ac 23/1.90-2.05
bDGlcpN 102.49/4.68 56.92/3.64 78.63/3.79 69.48/3.49 76.52/3.42 61.77/3.73-3.93
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,2 | Ac |
| 1.90
2.05 | |
| 3,3,4,2 | Ac |
| 1.90
2.05 | |
| 3,3,4 | aLFucpN | 5.52 | 4.10 | 3.89 | 3.77 | 3.94 | 1.19 |
| 3,3 | aDGalpNA | 5.14 | 4.43 | 4.18 | 4.71 | 4.49 |
|
| 3,2 | Ac |
| 1.90
2.05 | |
| 3 | aLFucpN | 4.89 | 4.31 | 4.01 | 3.73 | 4.40 | 1.15 |
| 2 | Ac |
| 1.90
2.05 | |
| | bDGlcpN | 4.68 | 3.64 | 3.79 | 3.49 | 3.42 | 3.73
3.93 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,2 | Ac | 173
175 | 23 | |
| 3,3,4,2 | Ac | 173
175 | 23 | |
| 3,3,4 | aLFucpN | 97.36 | 50.27 | 68.32 | 72.00 | 67.87 | 16.26 |
| 3,3 | aDGalpNA | 99.07 | 49.07 | 77.23 | 74.48 | 71.41 | 173
175 |
| 3,2 | Ac | 173
175 | 23 | |
| 3 | aLFucpN | 98.24 | 49.22 | 73.90 | 72.00 | 67.53 | 15.96 |
| 2 | Ac | 173
175 | 23 | |
| | bDGlcpN | 102.49 | 56.92 | 78.63 | 69.48 | 76.52 | 61.77 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
