Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 29352878Publication DOI: 10.1016/j.carbpol.2017.12.020Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Cordeiro LMC <lucimaramcc
ufpr.br>; Hamaker BR <hamakerb
purdue.edu>
Institutions: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Brazil, Whistler Center for Carbohydrate Research and Department of Food Science, Purdue University, West Lafayette, USA, Division of Glycoscience, School of Biotechnology, AlbaNova University Centre, KTH Royal Institute of Technology, Stockholm, Sweden, University of Florida, Gulf Coast Research and Education Center (GCREC-UF), Wimauma, USA
Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition. We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model. Two glucans, characterized as a (1→3),(1→6)-linked and a (1→3)-linked β-D-glucans were obtained. Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides. Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed. The (1→3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1→3),(1→6)-linked β-D-glucan. Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
Glucans, butyrate, Clostridium cluster XIVa, Cookeina speciose, in vitro fecal fermentation
Structure type: homopolymer
Location inside paper: p.17(Accepted manuscript), paragraph 2, Glucan B, Fig.2(B)
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, methylation, PCR, DNA sequencing, GC-MS, Smith degradation, biological assays, NaBH4 reduction, SEC, dialysis, DEPT, centrifugation, TFA hydrolysis
Comments, role: product of Smith degradation
Related record ID(s): 48818
NCBI Taxonomy refs (TaxIDs): 165092Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.2 73.1 86.4 68.6 76.6 61.1
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.2 | 73.1 | 86.4 | 68.6 | 76.6 | 61.1 |
|
There is only one chemically distinct structure:
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