Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 29924142Publication DOI: 10.1590/0074-02760180102Journal NLM ID: 7502619Publisher: Rio de Janeiro: Instituto Oswaldo Cruz
Correspondence: Santos ALSD <andre
micro.ufrj.br>
Institutions: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Microbiologia Geral, Laboratório de Estudos Avançados em Microrganismos Emergentes e Resistentes, Rio de Janeiro, Brasil, Universidade Federal do Rio de Janeiro, Instituto de Química, Programa de Pós-Graduação em Bioquímica, Rio de Janeiro, Brasil, Centro Universitário Estadual da Zona Oeste, Laboratório de Tecnologia em Cultura de Células, Rio de Janeiro, Brasil
Scedosporium/Lomentospora species are opportunistic mould pathogens, presenting notable antifungal resistance. We analysed the conidia and germinated conidia of S. apiospermum (Sap), S. aurantiacum (Sau), S. minutisporum (Smi) and L. prolificans (Lpr) by scanning electron microscopy and exposition of surface molecules by fluorescence microscopy. FINDINGS Conidia of Sap, Smi and Sau had oval, ellipsoidal and cylindrical shape, respectively, with several irregularities surrounding all surface areas, whereas Lpr conidia were rounded with a smooth surface. The germination of Sap occurred at the conidial bottom, while Smi and Sau germination primarily occurred at the centre of the conidial cell, and Lpr germination initiated at any part of the conidial surface. The staining of N-acetylglucosamine-containing molecules by fluorescein-labelled WGA primarily occurred during the germination of all studied fungi and in the conidial scars, which is the primary location of germination. Calcofluor white, which recognises the polysaccharide chitin, strongly stained the conidial cells and, to a lesser extent, the germination. Both mannose-rich glycoconjugates (evidenced by fluoresceinated-ConA) and cell wall externally located polypeptides presented distinct surface locations and expression according to both morphotypes and fungal species. In contrast, sialic acid and galactose-containing structures were not detected at fungal surfaces. MAIN CONCLUSIONS The present study demonstrated the differential production/exposition of surface molecules on distinct morphotypes of Scedosporium/Lomentospora species.
glycoconjugates, polysaccharides, cell surface, Scedosporium/Lomentospora, germination, polypeptides
Structure type: structural motif or average structure
Location inside paper: Abstract
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: statistical analysis, fluorescence microscopy, SEM, fluorescent staining of cell-wall
Comments, role: forms the main skeleton of the fungal cell wall; lower levels of chitin promotes increased sensitivity to stress factors
Related record ID(s): 48634, 48636, 48650, 48651, 48652, 48689, 48691, 48694, 48715, 48752, 48757, 48764, 48769, 48785, 48794, 48795, 48801, 48804, 48828, 48853, 48859, 48872, 48900, 48902, 48984
NCBI Taxonomy refs (TaxIDs): 563466,
315946,
315945,
41688Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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