Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 30449357Publication DOI: 10.1016/j.funbio.2018.09.005Journal NLM ID: 101524465Publisher: Amsterdam: Elsevier
Correspondence: Sakamoto Y <sakamoto
ibrc.or.jp>
Institutions: Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan, New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan, Iwate Biotechnology Research Center, Kitakami, Japan, TSUMURA and Co., Ami-machi, Japan, Facility of Engineering Graduate School of Engineering, Iwate University, Center for Regional Collaboration in Research and Education Electron Microscope Laboratory, Japan, School of Agriculture, Utsunomiya University, Utsunomiya, Japan
Lactase1 (Lcc1 ) is abundantly secreted from vegetative mycelia into culture medium by Lentinula edodes. Down-regulation of lcc1 in L edodes results in abnormal hyphal structure and thinner cell wall in mycelia. In this study, we observed the effects of lcc1 on the hyphal morphology and cell wall structure of L edodes. A thick cell wall and fibrous layer were clearly observed in the Icc1-silenced strain ivrL1#32, when purified Lcc1 (0.1 mU/mL) was added to the culture medium. The ratio of cell wall polysaccharide contents was compared between the ivrL1#32 strain and the wild-type (WT) strain SR-1, revealing that levels of the alkali soluble β-1,3-1,6-glucan were significantly lower in the lcc1-silenced strain than in the WT strain. Chronological analysis revealed that chitin content in the cell wall did not increase over time, but that the alkali soluble β-1,3-1,6-glucan content increased after Lccl secretion in the WT. Taken together, these data suggest that the increased level of β-1,3-1,6-glucan induced by Lcc1 in the mycelial cell wall contributes to increased cell wall thickness and strength.
cell wall polysaccharides, 6-glucan, 3-1, chitin, beta-1, white rot fungi, Basidiomycota
Structure type: structural motif or average structure
Location inside paper: Introduction
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: Western blotting, SEM, TEM, fluorescent staining of cell-wall
Comments, role: amount of chitin in cell wall is not significantly different between WT and silenced strain; chitin structure is not described in article
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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