Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wallAssociated disease: infection due to Cryptococcus neoformans [ICD11:
XN3EH 
]
NCBI PubMed ID: 32743128Publication DOI: 10.1016/j.tcsw.2018.05.002Journal NLM ID: 101728565Publisher: Amsterdam: Elsevier
Correspondence: Rodrigues ML <marcio.rodrigues
fiocruz.br>
Institutions: Instituto de Microbiologia Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, Instituto de Biofísica Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, Centro de Desenvolvimento Tecnológico em Saúde (CDTS) da Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil
Cryptococcus neoformans mutants lacking each of the eight putative chitin synthase genes (CHS) have been previously generated. However, it is still unclear how deletion of chitin synthase genes affects the cryptococcal capsule. Since the connections between chitin metabolism and capsular polysaccharides in C. neoformans are numerous, we analyzed the effects of deletion of CHS genes on capsular and capsule-related structures of C. neoformans. CHS deletion affected capsular morphology in multiple ways, as determined by scanning electron microscopy and immunofluorescence analysis. Molecular diameter, serological reactivity and export of capsular polysaccharide were also affected in most of the chsΔ mutants, but the most prominent alterations were observed in the chs3Δ strain. C. neoformans cells lacking CHS genes also had altered formation of extracellular vesicles and variable chitinase activity under stress conditions. These results reveal previously unknown functions of CHS genes that greatly impact the physiology of C. neoformans.
structure, capsule, chitin synthase, Cryptococcus meoformans
Structure type: structural motif or average structure
Location inside paper: Introduction, paragraph 2
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: ELISA, immunofluorescence analyses, fluorescence microscopy, dynamic light scattering, flow cytometry, SEM
Comments, role: important for cell integrity; interacts with GMX to form hybrid, immunologically activce glycan complexes
NCBI Taxonomy refs (TaxIDs): 235443Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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