Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: infection due to Candida [ICD11:
XN3CL 
]
NCBI PubMed ID: 29248112Publication DOI: 10.1016/j.funbio.2017.09.007Journal NLM ID: 101524465Publisher: Amsterdam: Elsevier
Correspondence: Shibata N <nshibata
tohoku-mpu.ac.jp>
Institutions: Medical Mycology Research Center, Chiba University, Chiba, Japan, Department of Infection and Host Defense, Tohoku Medical and Pharmaceutical University, Sendai, Japan, Department of Electronic and Information Engineering, Suzuka National College of Technology, Mie, Japan
Candida glabrata is the second most common source of Candida infections in humans. In this pathogen, the maintenance of cell wall integrity (CWI) frequently precludes effective pharmacological treatment by antifungal agents. In numerous fungi, cell wall modulation is reported to be controlled by endoplasmic reticulum (ER) stress, but how the latter affects CWI maintenance in C. glabrata is not clearly understood. Here, we characterized a C. glabrata strain harboring a mutation in the CNE1 gene, which encodes a molecular chaperone associated with nascent glycoprotein maturation in the ER. Disruption of cne1 induced ER stress and caused changes in the normal cell wall structure, specifically a reduction in the β-1,6-glucan content and accumulation of chitin. Conversely, a treatment with the typical ER stress inducer tunicamycin up-regulated the production of cell wall chitin but did not affect β-1,6-glucan content. Our results also indicated that C. glabrata features a uniquely evolved ER stress-mediated CWI pathway, which differs from that in the closely related species Saccharomyces cerevisiae. Furthermore, we demonstrated that ER stress-mediated CWI pathway in C. glabrata is also induced by the disruption of other genes encoding proteins that function in a correlated manner in the quality control of N-linked glycoproteins in the ER. These results suggest that calcineurin and ER quality control system act as a platform for maintaining CWI in C. glabrata.
chitin, Candida glabrata, cell wall integrity, calnexin, ER stress, cne1
Structure type: structural motif or average structure
Location inside paper: Abstract
Trivial name: chitin
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: acid hydrolysis, statistical analysis, flow cytometry, centrifugation, differential interference contrast microscopy, Ehrlich test
Comments, role: CgCNE1 disruption or tunicamycicn treatment of cell culture results in cell wall chitin accumulation; ER stress induces cell wall chitin up-regulation and cell agglutination; cell wall chitin is required for cell survival under ER stress
NCBI Taxonomy refs (TaxIDs): 5478Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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(previously named: Cryptococcus glabratus KUE100)