Taxonomic group: bacteria / Chlamydiae
(Phylum: Chlamydiae)
Associated disease: infection due to Chlamydia [ICD11:
XN27H 
]
NCBI PubMed ID: 9729792Publication DOI: 10.1023/a:1016047602190Journal NLM ID: 9110829Publisher: ESCOM Science Publishers
Institutions: Institut fur Chemie der Universitat fur Bodenkultur Wien, A-1190 Wien, Austria, Institut fur Chemie,MedizinischeUniversitat Lubeck, Ratzeburger Allee 160, D-23538 Lubeck, Germany, Bruker Analytik GmbH, Silberstreifen, D-76287 Rheinstetten, Germany, Forschungszentrum Borstel, Zentrum fur Medizin und Biowissenschaften Borstel, Germany
The disaccharide α-Kdo-(2→8)-α-Kdo (Kdo: 3-deoxy-D-manno-oct- 2-ulosonic acid) represents a genus-specific epitope of the lipopolysaccharide of the obligate intracellular human pathogen Chlamydia. The conformation of the synthetically derived disaccharide α-Kdo-(2→8)-α-Kdo-(2→O)-allyl was studied in aqueous solution, and complexed to a monoclonal antibody S25-2. Various NMR experiments based on the detection of NOEs (or transfer NOEs) and ROEs (or transfer ROEs) were performed. A major problem was the extensive overlap of almost all 1H NMR signals of α-Kdo-(2→8)-α-Kdo-(2→O)-allyl. To overcome this difficulty, HMQC-NOESY and HMQC-trNOESY experiments were employed. Spin diffusion effects were identified using trROESY experiments, QUIET-trNOESY experiments and MINSY experiments. It was found that protein protons contribute to the observed spin diffusion effects. At 800 MHz, intermolecular trNOEs were observed between ligand protons and aromatic protons in the antibody binding site. From NMR experiments and Metropolis Monte Carlo simulations, it was concluded that α-Kdo-(2→8)-α-Kdo-(2→O)-allyl in aqueous solution exists as a complex conformational mixture. Upon binding to the monoclonal antibody S25-2, only a limited range of conformations is available to α-Kdo-(2→8)-α-Kdo-(2→O)-allyl. These possible bound conformations were derived from a distance geometry analysis using transfer NOEs as experimental constraints. It is clear that a conformation is selected which lies within a part of the conformational space that is highly populated in solution. This conformational space also includes the conformation found in the crystal structure. Our results provide a basis for modeling studies of the antibody- disaccharide complex.
Kdo, monoclonal antibody, HMQC-trNOESY, QUIET-trNOESY, trNOESY, trROESY
Structure type: oligomer
Location inside paper: abstract
Trivial name: disaccharide a-(2-8)-linked Kdo
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_130658
Methods: NMR-2D
Biological activity: binding to the monoclonal antibody S25-2
3D data: conformation data, Monte carlo simulations, conformation analysis
Related record ID(s): 565, 3235, 3880, 4143, 22428, 116695
NCBI Taxonomy refs (TaxIDs): 810Reference(s) to other database(s): GlycomeDB:
5769
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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