Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelium
NCBI PubMed ID: 16102810Publication DOI: 10.1016/j.ijbiomac.2005.06.003Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Gao X <xiangdong_gao
hotmail.com>
Institutions: School of Life Science and Technology, China Pharmaceutical University, Nanjing, China, School of Life Science, Nanjing University, Nanjing, China
A polysaccharide YCP was prepared from a marine filamentous fungus Keissleriella sp. YS4108, which exhibited as a molecular weight (Mw) of 2400 kDa and its three sulfated derivatives (YCP-SL, YCP-SM and YCP-SH) were synthesized, the degree of substitution (DS) of which were determined to be 0.13, 0.99 and 1.3, with the average molecular weight 640, 570 and 450 kDa, respectively. Anticoagulant activity and antiplatelet aggregation activity of these sulfated derivates were evaluated by activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and platelet aggregation assay. The results showed that YCP sulfates significantly prolonged APTT, TT and PT. The derivates showed no effects on thrombin in the presence or in the absence of antithrombin III (AT III) or heparin cofactor II (HC II), while the derivates effectively inhibited factor Xa in the presence of AT III. At the same time, YCP-SH also possessed potent antiplatelet aggregation activity in vitro compared with aspirin. YCP sulfates specifically interfered with different stages of the coagulation cascade, and the anticoagulant activity improved with the increasing DS and decreased Mw
sulfation, anticoagulant activity, Marine polysaccharide, antiplatelet aggregation activity
Structure type: homopolymer ; 2400000
Location inside paper: p. 203, right column, paragraph 1, Table 2, YCP
Trivial name: α-(1,4)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, IR, biological assays, GPC, extraction, dialysis, anticoagulation activity, precipitation, derivatization, sonication
Comments, role: Keissleriella sp. YS 4108 error
Related record ID(s): 49220, 49222, 49223, 50866
NCBI Taxonomy refs (TaxIDs): 73001Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 103.536 74.413 76.093 80.690 72.071 63.298
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 103.536 | 74.413 | 76.093 | 80.690 | 72.071 | 63.298 |
|
There is only one chemically distinct structure:
Found 
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