Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 19885951Publication DOI: 10.1142/S0192415X09007351Journal NLM ID: 7901431Publisher: Singapore; River Edge, NJ: World Scientific Pub
Correspondence: Liu JC <qyybliu
126.com>
Institutions: Institute of Medicine, Qiqihar Medical College, Qiqihar, China
The components of Agaricus blazei Murill (AbM) have been shown to possess antitumor potentials. Herein, we attempted to explore the anti-metastatic effect and underlying mechanism of a low molecular weight polysaccharide isolated from AbM (LMPAB). Matrigel invasion assay was applied to evaluate the effect of LMPAB on migration of BEL-7402 hepatic cancer cells in vitro. In vivo, the anti-metastatic effect of LMPAB was investigated in mouse B16 melanoma and a double-grafted SW180 tumor models. mRNA and protein levels of metalloproteinase-9 (MMP-9) or nm23-H1 upon LMPAB treatment were detected by real-time PCR and immunohistochemistry assays. LMPAB significantly reduced the invasion of BEL-7402 cells. In vivo, LMPAB was revealed to decrease lung metastatic foci in mouse B16 melanoma model. In the double-grafted SW180 mouse tumor model, we further demonstrated that intratumoral treatment of LMPAB inhibited the growth of tumor on treated side but also suppresses the regression of metastatic tumors on the non-treated side. Moreover, LMPAB reduced MMP-9 but enhanced nm23-H1 mRNA and protein expression. LMPAB displays anti-metastatic activities, indicating the potential of its clinical application for the prevention and treatment of cancer metastasis. Its anti-metastatic effect may relate to the modulation on MMP-9 and nm23-H1
polysaccharide, Agaricus blazei, anti-metastasis, metalloproteinase-9, nm23-H1
Structure type: homopolymer ; 48000
Location inside paper: p. 911, paragraph 3
Trivial name: β-(1,3)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, extraction, RT-PCR, HPGPC, immunohistochemistry
Biological activity: glucan inhibits tumor metastasis both in vitro and in vivo. The inhibition of MMP-9 and promotion of nm23-H1-dependent signaling pathways are at least partially responsible for anti-metastatic effects of glucan
Related record ID(s): 3459, 42138, 42290, 42294, 43554, 47304, 47707, 48305, 48371, 48410, 48411, 48472, 49257, 49258, 49266, 49282, 49290, 102752
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
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