Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 19082455Publication DOI: 10.3892/or_0000201Journal NLM ID: 9422756Publisher: Athens: D.A. Spandidos
Correspondence: Liu JC <qyybliu
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Institutions: Institute of Medicine, Qiqihar Medical College, Qiqihar, China, Department of Urology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Previous studies indicated that the low molecular weight polysaccharide extracts from Agaricus blazei are potential antitumor agents or adjuvant in tumor treatment. In this study, we investigated the antitumor activity of LMPAB, a low molecular weight polysaccharide isolated from Agaricus blazei, and the molecular mechanisms of its antitumor activity. The antitumor effect of LMPAB was examined using mouse sarcoma 180 (S180) xenograft models. Antiangiogenic effect of LMPAB was determined by chicken embryo chorioallantoic membrane (CAM) angiogenesis and Matrigel-induced neovascularization in vivo models. The mRNA and protein levels of vascular endothelial growth factor (VEGF) were assessed using real-time reverse transcription-polymerase chain reaction, immunohistochemistry, and enzyme-linked immunosorbent assays. Tumor inhibitory rates in the S180 xenograft models were 9.7, 23.9, and 33.0%, respectively, after administration of LMPAB at dose of 50, 100, and 200 mg/kg/day for 2 weeks. LMPAB also inhibited angiogenesis in the CAM model and Matrigel-induced neovascularization in C57BL/6 mice. The mRNA and protein levels of VEGF in tumor tissues were significantly down-regulated in the BALB/c mice received LMPAB treatment. Furthermore, significant down-regulation of serum VEGF levels was also observed in the mice. Our data suggest that LMPAB might be a promising agent for tumor therapy, and the antitumor and antiangiogenic effects of LMPAB may be related with down-regulation of VEGF
antitumor, Agaricus blazei, antiangiogenesis, vascular endothelial growth factor, low molelular weight polysaccharide
Structure type: homopolymer ; 48000
Location inside paper: p. 146, left column, paragraph 1
Trivial name: β-(1,3)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: ELISA, biological assays, UV, extraction, RT-PCR, HPGPC, antitumor activity assay, immunohistochemistry
Biological activity: glucan has significant antitumor and antiangiogenic effects in vivo. Glucan also has a down-regulating effect on VEGF expression in tumor tissues. Furthermore, serum VEGF levels in the tumor-bearing BALB/c mice were significantly downregulated by glucan treatment
Related record ID(s): 3459, 42138, 42290, 42294, 43554, 47304, 47707, 48305, 48371, 48410, 48411, 48472, 49256, 49258, 49266, 49282, 49290, 102752
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
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