Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 25919894Publication DOI: 10.1002/cbic.201500103Journal NLM ID: 100937360Publisher: Weinheim, Germany: Wiley Interscience
Correspondence: Yamaguchi Y <yyoshiki
riken.jp>
Institutions: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany, Structural Glycobiology Team, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, RIKEN Global Research Cluster, Wako, Japan, Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany, Glycosciences Laboratory, Department of Medicine, Imperial College London, London, UK, The Glycoscience Institute, Ochanomizu University, Tokyo, Japan, Disease Glycomics Team, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, RIKEN Global Research Cluster, Wako, Japan
ZG16p is a soluble mammalian lectin that interacts with mannose and heparan sulfate. Here we describe detailed analysis of the interaction of human ZG16p with mycobacterial phosphatidylinositol mannosides (PIMs) by glycan microarray and NMR. Pathogen-related glycan microarray analysis identified phosphatidylinositol mono- and di-mannosides (PIM1 and PIM2) as novel ligand candidates of ZG16p. Saturation transfer difference (STD) NMR and transferred NOE experiments with chemically synthesized PIM glycans indicate that PIMs preferentially interact with ZG16p by using the mannose residues. The binding site of PIM was identified by chemical-shift perturbation experiments with uniformly 15N-labeled ZG16p. NMR results with docking simulations suggest a binding mode of ZG16p and PIM glycan; this will help to elucidate the physiological role of ZG16p
NMR spectroscopy, chemical synthesis, lectins, carbohydrate microarrays, microarrays, phosphatidyl inositol mannoside
Structure type: homopolymer
Location inside paper: Table 1, 20
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, DNA cloning, DNA techniques, chemical synthesis, enzymatic digestion, ion-exchange chromatography, molecular modeling, 15N NMR, SEC, CC, binding assays, cell growth, gene expression, sonication, centrifugation
Biological activity: no significant binding is observed for ZG16p to the pustulan
Related record ID(s): 7724, 8325, 42140, 43301, 43379, 44888, 44927, 44939, 45578, 47842, 47843, 48303, 48366, 48412, 48423, 48443, 49289, 49290, 49292, 49293, 49294, 101372, 124916, 138263, 143680, 143682, 149870
NCBI Taxonomy refs (TaxIDs): 136371Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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