Capsular polysaccharides were recovered from four Acinetobacter baumannii isolates, and the following related structures of oligosaccharide repeating units were established by sugar analyses along with 1D and 2D 1H and 13C NMR spectroscopy: NIPH 60 and LUH5544 (K43) NIPH 601 (K47) The K locus for capsule biosynthesis in the genome sequences available for NIPH 60 and LUH5544, designated KL43, was found to be related to gene clusters KL47 in NIPH 601 and KL88 in LUH5548. The three clusters share most gene content differing in only a small portion that includes an additional glycosyltransferase genes in KL47 and KL88, as well as genes encoding distinct Wzy polymerases that were found to form the same α-d-GlcpNAc-(1→6)-α-d-GlcpNAc linkage in K43 and K47.

Acinetobacter baumannii, capsular polysaccharide, glycosyltransferase, Wzy polymerase, K locus

Publication DOI: 10.1016/j.carres.2016.10.007
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: yknirelgmail.com
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, M.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russi, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, chemical analysis, acid hydrolysis, GLC, NMR-1D, bioinformatic analysis
 

• Compound ID: 2078
  

  b-D-Glcp-(1-3)-+ | -6)-a-D-GlcpNAc-(1-4)-a-D-GalpNAc-(1-3)-a-D-GlcpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Trivial name: surface polysaccharide
  Compound class: CPS, O-polysaccharide, O-antigen
  Reference(s) to other database(s): GTC:G53088UD, GlycomeDB:25207
  
   
  
  Acinetobacter baumannii NIPH 60 (K43), Acinetobacter baumannii LUH5544
  CSDB ID 11361 (all data & tools)
• Compound ID: 12451
  

  b-D-Glcp-(1-3)-+ | -6)-a-D-GlcpNAc-(1-6)-a-D-GlcpNAc-(1-4)-a-D-GalpNAc-(1-3)-a-D-GlcpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  Reference(s) to other database(s): GTC:G51712EK
  
   
  
  Acinetobacter baumannii NIPH 601 (K47)
  CSDB ID 11791 (all data & tools)
• Compound ID: 12452
  

  -2)-b-D-Glcp-(1-6)-a-D-GlcpNAc-(1-6)-a-D-GlcpNAc-(1-4)-a-D-GalpNAc-(1-3)-b-D-GlcpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  Reference(s) to other database(s): GTC:G78379FN
  
   
  
  Acinetobacter baumannii LUH5548 (K88)
  CSDB ID 11792 (all data & tools)
• Compound ID: 12031
  

  b-D-Glcp-(1-3)-+ | -4)-a-D-GalpNAc6(50%)Ac-(1-4)-a-D-GalpNAc-(1-3)-a-D-GlcpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  Reference(s) to other database(s): GTC:G57327XB
  
   
  
  Acinetobacter baumannii K45
  CSDB ID 11793 (all data & tools)
• Compound ID: 12029
  

  b-D-Glcp-(1-3)-+ | -3)-a-D-Glcp-(1-6)-a-D-GlcpNAc-(1-4)-a-D-Galp-(1-3)-b-D-GlcpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  Reference(s) to other database(s): GTC:G35428RP
  
   
  
  Acinetobacter baumannii K48
  CSDB ID 11794 (all data & tools)
• Compound ID: 12028
  

  b-D-Glcp-(1-3)-+ | -2)-a-D-Galp-(1-6)-a-D-Glcp-(1-4)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: CPS
  Reference(s) to other database(s): GTC:G68883RH
  
   
  
  Acinetobacter baumannii K30
  CSDB ID 11795 (all data & tools)