Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: mycelium
NCBI PubMed ID: 31004649Publication DOI: 10.1016/j.ijbiomac.2019.04.116Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: lyadour
gmail.com
Institutions: Department of Chemistry, Faculty of Sciences, University Mouloud MAMMERI, Tizi-Ouzou, Algeria, Laboratory of analytical biochemistry and biotechnology, University Mouloud MAMMERI, Tizi-Ouzou, Algeria, Department of Chemistry, Faculty of Sciences, University Algiers, Algiers, Algeria, Bioengineering et Génie des Procédés (BIOGEP), Ecole Nationale Polytechnique, El Harrach, Algeria, Université de Rennes, Ecole Nationale Supérieure de Chimie de Rennes, Rennes, France
This study was devoted to polysaccharides extraction (chitin and chitosan) from Penicillium camembertii cell wall. A culture on solid medium was adopted under carefully selected conditions, appropriate to mycelium growth: duration 6 days, medium YPGA and pH 5. The temperature was adjusted (20 °C to 28 °C) in order to study the effect of temperature on chitin/chitosan production. Biomass decreased with increasing temperatures: 13 g/L at 20 °C and 11.6 g/L at 28 °C. For all tested temperatures, the yields of insoluble alkaline fractions (AIM) were almost identical (200 mg/g). The solubility of fractions in 2% acetic acid allowed obtaining two fractions: an insoluble fraction (AcIM) with 18% of maximum yield and soluble fraction (AcSM) with 1% yield. The SEM micrographs of AcIM fractions were similar to AIM fractions. These showed a compact structure different from commercial chitin. The presence of chitin in P. Camembertii cultured in YPGA medium was also confirmed by ATR spectroscopy.
chitin, Biomass, free chitosan, Penicillium camembertii, YPGA
Structure type: structural motif or average structure
Location inside paper: p. 1000, Fig. 1, chitosan
Trivial name: chitosan
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: IR, extraction, cell growth, SEM, centrifugation, optical microscopy
Synthetic data: enzymatic in vivo
Comments, role: the degree of deacetylation of the chitosan extracted after cultivation at 28 °C was above 50%
Related record ID(s): 41553, 41561, 44856, 44877, 44886, 46311, 48638, 48760, 48765, 48774, 48805, 49512, 49524, 49653, 50016, 50301, 50303, 50304, 50307, 50308, 50310, 50311, 50314, 50315, 50317, 50319, 50320
NCBI Taxonomy refs (TaxIDs): 5075Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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