Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus flavus [ICD11:
XN6B8 
]
NCBI PubMed ID: 30747436Publication DOI: 10.1002/jobm.201800545Journal NLM ID: 8503885Correspondence: Jia Y <jiayingmin
btbu.edu.cn>
Institutions: Lab of Enzyme Engineering, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China
A novel xylanase from the filamentous fungus Aspergillus flavus was purified and characterized as the β-1,4-endoxylanase (designed as AfXynB) with a molecular mass (32.2 kDa), which is different from all of the previously reported xylanases from the same strain. AfXynB was optimally active at pH 7.5 and 55 °C, respectively. It was stable up to 50 °C within range of pH 4.0-9.5, and displayed an excellent tolerance to various cations, reagents, and proteases. AfXynB showed specific activity toward beechwood xylan but no detected activity toward CMC and pNP-β-D-xylopyranoside. The xylanase is a typical endo-xylanase; it could hydrolyze beechwood xylan to only yield xylobiose (X2) and xylopentaose (X5). Actually, this may be the first report for the endo-xylanases that displayed such a unique hydrolytic property. These findings in the present study have great implications for its future applications of the novel xylanase
purification, β-1, biochemical properties, Aspergillus flavus, xylooligosaccharides, 4-endoxylanase
Structure type: oligomer
Location inside paper: Fig. 3, xylopentaose
Trivial name: xylopentaose
Contained glycoepitopes: IEDB_114701,IEDB_167188,IEDB_174332
Methods: SDS-PAGE, enzymatic digestion, extraction, CC, cell growth, enzymatic assay, precipitation, spectrophotometry, centrifugation, dinitrosalicylic acid (DNS) method, Lowry method
Enzymes that release or process the structure: β-1,4-endoxylanase (AfXynB)
Synthetic data: enzymatic
Related record ID(s): 49516, 49517, 49518, 49519
NCBI Taxonomy refs (TaxIDs): 5059Reference(s) to other database(s): GTC:G47101GE
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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