Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
NCBI PubMed ID: 30766929Publication DOI: 10.1016/j.heliyon.2019.e01173Journal NLM ID: 101672560Publisher: London: Elsevier Ltd
Correspondence: Latgé JP <jplatge
pasteur.fr>; Nifantiev NE <nen
ioc.ac.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Unité des Aspergillus, Institut Pasteur, Paris, France
Great progresses have been made in the recent years in the detection of circulating galactofuranose-bearing molecules for the diagnosis of aspergillosis. However, the test used in the clinical practice is hampered by the occurrence of false positives. A glycoarray with dozens of oligosaccharides structurally related to the Aspergillus fumigatus galactomannan has allowed us to reinvestigate the carbohydrate specificity of the EB-A2 monoclonal antibody used in the PlateliaTM Aspergillus sandwich immune assay. We have now demonstrated that the mAb can recognize shorter oligosaccharides than the previously reported tetrasaccharide Galf-β-(1→5)-Galf-β-(1→5)-Galf-β-(1→5)-Galf-β and oligosaccharides which contains alternating β-(1→5)/β-(1→6)-linkages. This result could explain the occurrence of false-positive signals due to the presence of the abovementioned epitopes not only in A. fumigatus galactomannan but also in other bacteria and fungi.
microbiology, immunology, infectious disease, biochemistry, health sciences
Structure type: oligomer
Location inside paper: Fig. 1, A, 11
Compound class: O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136095,IEDB_137472,IEDB_137485,IEDB_144983,IEDB_149137,IEDB_152206,IEDB_190606,IEDB_885812,IEDB_983930,SB_44,SB_67,SB_72
Methods: ELISA, binding assay
Related record ID(s): 49845, 49846, 49847, 49848, 49849, 49850, 49851, 49852, 49853, 49855, 49856
NCBI Taxonomy refs (TaxIDs): 746128
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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