Ota R, Okamoto Y, Vavricka CJ, Oka T, Matsunaga E, Takegawa K, Kiyota H, Izumi M Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan Carbohydrate Research473 (2019)
99-103
NCBI PubMed ID:30658252 Publication DOI:10.1016/j.carres.2019.01.005 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: mizumiokayama-u.ac.jp Institutions: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan, Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan, Graduate School of Science, Technology and Innovation, Kobe University, Nada, Japan
β-D-galactofuranose (Galf) is a component of polysaccharides and glycoconjugates. There are few reports about the involvement of galactofuranosyltransferases and galactofuranosidases (Galf-ases) in the synthesis and degradation of galactofuranose-containing glycans. The cell walls of filamentous fungi in the genus Aspergillus include galactofuranose-containing polysaccharides and glycoconjugates, such as O-glycans, N-glycans, and fungal-type galactomannan, which are important for cell wall integrity. In this study, we investigated the synthesis of p-nitrophenyl β-D-galactofuranoside and its disaccharides by chemo-enzymatic methods including use of galactosidase. The key step was selective removal of the concomitant pyranoside by enzymatic hydrolysis to purify p-nitrophenyl β-D-galactofuranoside, a promising substrate for β-D-galactofuranosidase from Streptomyces species.
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