Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 31320072Publication DOI: 10.1016/j.carbpol.2019.114990Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: mondalsoumitra78
yahoo.com
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, India, Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, India, Department of Microbiology, Panskura Banamali College, Panskura, India, Department of Chemistry, Panskura Banamali College, Panskura, India, Department of Biotechnology, Panskura Banamali College, Panskura, India, Department of Chemistry, Pingla Thana Mahavidyalaya, Maligram, India
A water insoluble β-glucan (PS), with molecular mass ∼91600 Da was isolated from the 4% alkaline extract of an edible mushroom, Pleurotus djamor and found to consist of (1→3)-β-D-glucopyranosyl moiety. The structure of the PS was elucidated on the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (1H, 13C, DQF-COSY, DEPT-135, and HSQC). The structure of the repeating unit of the polysaccharide was established as: →3)-β-D-Glcp-(1→. The water insoluble β-glucan showed cytotoxic effect against PA1 cells, where˜50% population was destroyed at 100 μg/mL concentration, and almost all cells at 250 μg/mL concentration. The wound healing assay showed significant anticarcinogenic effect against ovarian carcinoma PA1 cells after 48 h of treatment.
β-glucan, NMR experiments, Edible mushroom, cytotoxic effect, wound healing assay
Structure type: homopolymer ; 91600
Location inside paper: paragraph 3.3.2, structure 1, Table 1
Trivial name: β-(1,3)-glucan, curdlan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, acid hydrolysis, GLC, Smith degradation, biological assays, paper chromatography, extraction, acetylation, methylation analysis, reduction, CC, dialysis, cell viability assay, precipitation, phenol-sulfuric acid assay, spectrophotometry, derivatization, Gerwig method, centrifugation, MTT
Biological activity: glucan showed a specific cytotoxic effect against PA1 cells, where almost all cells was destroyed at 250 μg/mL concentration and also this glucan exhibited significant anti-proliferation against ovarian carcinoma PA1 cells and was nontoxic against healthy HEK 293 cells
Related record ID(s): 41552, 41631, 41632, 50036, 50617, 50648, 50654, 50665, 50671, 50861
NCBI Taxonomy refs (TaxIDs): 34470Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.5 73.3 86.6 68.8 76.8 61.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.50 3.28 3.46 3.21 3.24 3.45-3.68
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.5/4.50 73.3/3.28 86.6/3.46 68.8/3.21 76.8/3.24 61.4/3.45-3.68
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.50 | 3.28 | 3.46 | 3.21 | 3.24 | 3.45
3.68 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.5 | 73.3 | 86.6 | 68.8 | 76.8 | 61.4 |
|
There is only one chemically distinct structure:
Found 
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