Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1016/j.postharvbio.2019.04.021Journal NLM ID: 9884964Publisher: Amsterdam; New York: Elsevier
Correspondence: Yu T <yuting
zju.edu.cn>
Institutions: National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China
In this study, the (1→3)-β-D-glucan was isolated from yeast cell wall for investigating its effect on reducing the postharvest decay of pear fruit caused by Penicillium expansum and the underlying mechanisms. The results demonstrated that (1→3)-β-D-glucan could significantly induce disease resistance against P. expansum in pear wounds. (1→3)-β-D-glucan did not directly affect the growth of P. expansum in vitro and in vivo, whereas the spore germination of the pathogen was obviously inhibited in pear wounds when the induction time increased to 24 h by (1→3)-β-D-glucan. Furthermore, a large variety of defense-related genes, including PR1, GLU, endoGLU9, CHI4, endoCHI, PR4, PR5, CHI3 and PAL, were markedly up-regulated by (1→3)-β-D-glucan treatment. To the best of our knowledge, this is the first report that (1→3)-β-D-glucan can induce the disease resistance in the postharvest fruit. These findings suggest that application of (1→3)-β-D-glucan may be an effective strategy to reduce the fungal rot of the postharvest fruit.
(1→3)-β-D-glucan, induced resistance, Penicillium expansum, postharvest, pear fruit
Structure type: homopolymer ; 165000
Location inside paper: p. 110, Fig. 2
Trivial name: β-(1,3)-glucan, curdlan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, IR, acid hydrolysis, biological assays, HPLC, alkaline hydrolysis, viscosity measurement, extraction, RT-PCR, cell growth, gene expression, SEM, derivatization, centrifugation, qRT-PCR, optical microscopy
Biological activity: polysaccharide could induce disease resistance against P. expansum in postharvest pear fruit. Meanwhile, polysaccharide also remarkably up-regulated the expression of PR genes, which might be an important mechanism by which polysaccharide reduces the postharvest decay of pear fruit
Comments, role: solvent in 1H NMR experiments was DMSO-d6
Related record ID(s): 41552, 41631, 41632, 50617, 50648, 50654, 50665, 50671, 50861
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in DMSO-d6 / adamantane at 333 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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