Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 31083511Publication DOI: 10.3390/ijms20092337Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Theis TV <thaisvtheis
gmail.com>; Queiroz Santos VA <vidianyqueiroz
yahoo.com.br>; Appelt P <patriciaappelt18
gmail.com>; Barbosa-Dekker AM <anelibarbosa
gmail.com>; Vetvicka V <Vaclav.vetvicka
louisville.edu>; Dekker RFH <xylanase
gmail.com>; Cunha MAA <mcunha
utfpr.edu.br>
Institutions: Department of Pathology, University of Louisville, Louisville, USA, Centro de Ciências Exatas, Departamento de Química, Universidade Estadual de Londrina, Londrina, Brazil, Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Pato Branco, Brazil, Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Campo Mourão, Brazil, Programa de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do Paraná (UTFPR), Londrina, Brazil
Exocellular (1→6)-β-D-glucan (lasiodiplodan) produced by the fungus Lasiodiplodia theobromae MMPI was derivatized by carboxymethylation using different concentrations of a derivatizing agent. Lasiodiplodan was derivatized by carboxymethylation in an attempt to increase its solubility and enhance its biological activities. Carboxymethylglucans with degrees of substitution (DS) of 0.32, 0.47, 0.51, 0.58, and 0.68 were produced and characterized. FTIR analysis showed a band of strong intensity at 1600 cm-1 and an absorption band at 1421 cm-1, resulting from asymmetric and symmetrical stretching vibrations, respectively, of the carboxymethyl group COO- in the carboxymethylated samples. Thermal analysis showed that native lasiodiplodan (LN) and carboxymethylated derivatives (LC) exhibited thermal stability up to 200-210 °C. X-ray diffractometry demonstrated that both native and carboxymethylated lasiodiplodan presented predominantly an amorphous nature. Scanning electron microscopy revealed that carboxymethylation promoted morphological changes in the biopolymer and increased porosity, and alveolar structures were observed along the surface. The introduction of carboxymethyl groups in the macromolecule promoted increased solubility and potentiated the hydroxyl radical-scavenging activity, suggesting a correlation between degree of substitution and antioxidant activity.
carbohydrate, exopolysaccharide, biopolymer, lasiodiplodan, bioactive macromolecules
Structure type: homopolymer
Location inside paper: Fig. 1, A, left structure
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: IR, X-ray, cell growth, antioxidant activities, precipitation, SEM, derivatization, centrifugation, thermogravimetric analysis, titration
Related record ID(s): 50088
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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