Taxonomic group: fungi /
Organ / tissue: cell wall
NCBI PubMed ID: 32482674Publication DOI: 10.1128/AAC.00476-20Journal NLM ID: 0315061Correspondence: Ramage G <Gordon.Ramage
glasgow.ac.uk>; Brown JL <Jason.Brown
glasgow.ac>
Institutions: Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK, São Paulo State University (Unesp), School of Dentistry, Department of Preventive and Restorative Dentistry, São Paulo, Brazil, Glasgow Biofilm Research Network, Glasgow Dental School, Glasgow, UK, Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Glasgow, UK, Graduate Program in Dentistry, University of Western São Paulo (UNOESTE), Prudente/São Paulo, Brazil
Candida auris has emerged as a multidrug-resistant nosocomial pathogen over the last decade. Outbreaks of the organism in health care facilities have resulted in life-threatening invasive candidiasis in over 40 countries worldwide. Resistance by C. auris to conventional antifungal drugs such as fluconazole and amphotericin B means that alternative therapeutics must be explored. As such, this study served to investigate the efficacy of a naturally derived polysaccharide called chitosan against aggregative (Agg) and nonaggregative (non-Agg) isolates of C. auris in vitro and in vivo. In vitro results indicated that chitosan was effective against planktonic and sessile forms of Agg and non-Agg C. auris In a Galleria mellonella model to assess C. auris virulence, chitosan treatment was shown to ameliorate killing effects of both C. auris phenotypes (NCPF 8973 and NCPF 8978, respectively) in vivo Specifically, chitosan reduced the fungal load and increased survival rates of infected Galleria, while treatment alone was nontoxic to the larvae. Finally, chitosan treatment appeared to induce a stress-like gene expression response in NCPF 8973 in the larvae likely arising from a protective response by the organism to resist antifungal activity of the compound. Taken together, results from this study demonstrate that naturally derived compounds such as chitosan may be useful alternatives to conventional antifungals against C. auris.
aggregates, therapeutics, chitosan, Candida auris, Galleria mellonella
Structure type: structural motif or average structure
Location inside paper: abstract
Trivial name: chitosan
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: biological assays, cell growth, gene expression, DNA extraction, RNA extraction, SEM, TEM, centrifugation
Biological activity: chitosan has antifungal activity against a selection of Candida auris isolates with different phenotypes, both in vitro and in vivo. Chitosan appeared to induce a stress-like response in the nonaggregative Candida auris isolate compared to that in the aggregative isolate
Related record ID(s): 41553, 44877, 44886, 46311, 46570, 46683, 48760, 48774, 49133, 49502, 49512, 49524, 49653, 50016, 50301, 50303, 50307, 50308, 50310, 50311, 50314, 50315, 50317, 50319, 50320
NCBI Taxonomy refs (TaxIDs): 4751Reference(s) to other database(s): GTC:G97099AY
Show glycosyltransferases
There is only one chemically distinct structure:
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