Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 32333466Publication DOI: 10.1002/cbdv.202000175Journal NLM ID: 101197449Publisher: Verlag Helvetica Chimica Acta
Correspondence: Craciunescu O <oana_craciunescu2009
yahoo.com>; office
incdsb.ro
Institutions: National Institute of Research and Development for Biological Sciences, Bucharest, Romania, ''Petru Poni'' Institute of Macromolecular Chemistry, Iasi, Romania, National Institute for Research and Development in Chemistry and Petrochemistry - ICECHIM, Bucharest, Romania
Two chitosan extracts were prepared by chemical and enzymatic treatment of Ganoderma lucidum mushroom, as an alternative source to crustacean shells. The molecular weight of the enzymatic extract was lower than that of the chemical one and of shrimp chitosan, as determined by viscosity measurements. Characteristic signals were identified in the 1 H-NMR spectra and high deacetylation degree indicated good physico-chemical properties for both mushroom chitosan extracts. The scavenging capacity of mushroom chitosan extracts was moderate against the synthetic radicals of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), but higher values were observed for the enzymatic extract, compared to the chemical extract and shrimp chitosan. In vitro cytotoxicity was evaluated in L929 mouse fibroblast cell lines and the results of MTT assay showed good cytocompatibility in the tested range of concentrations. The growth of Gram-positive bacteria was inhibited more than Gram-negative bacteria in the presence of mushroom chitosan extracts, in particular by the chemical one, indicating their efficiency as antimicrobial agents. All these results strengthen the evidence of mushroom polysaccharide preparations availability for biomedical applications.
extraction, deacetylation, fungi, radical scavenging activity, α-amylase
Structure type: homopolymer
Location inside paper: Fig. 1, chitin
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: 1H NMR, alkaline hydrolysis, enzymatic digestion, extraction, antioxidant activities, cell viability assay, cytotoxicity assay, precipitation, antimicrobial assay, centrifugation, optical density measurement, deproteinization
Enzymes that release or process the structure: α-amylase
Related record ID(s): 40760, 40800, 41831, 49862, 50302, 50305, 50306, 50309, 50312, 50313, 50317, 50318, 111874, 121703, 131817, 139821, 143656, 147987, 149876
NCBI Taxonomy refs (TaxIDs): 5315Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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