Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus flavus [ICD11:
XN6B8 
]
Publication DOI: 10.1016/j.biteb.2020.100546Journal NLM ID: 101747079Publisher: Amsterdam: Elsevier Ltd.
Correspondence: romybarros
hotmail.com
Institutions: Laboratory of Flavor & Chromatographic Analysis, PROCTA, Federal University of Sergipe, São Cristóvão, Brazil
Eight strains of Aspergillus spp. were evaluated for their ability to produce intracellular and extracellular fructosyltransferase and fructooligosaccharides from sucrose. To the best of our knowledge, this is the first time that a study evaluates the production of intracellular and extracellular fructosyltransferase simultaneously with its chemometric characterization. High values of intracellular FTase were exhibited by A. oryzae and A. carbonarius and extracellular FTase by A. japonicus and A. ochraceus. The highest concentrations of intracellular FOS were generated by A. carbonarius and A. ochraceus and extracellular FOS by A. carbonarius and A. japonicus. The variations detected between the behavior of intra- and extra-cellular enzymes can be explained by activation of disulfide bridges and N-terminal signal peptides that occur distinctly between these biological structures. 1-kestose and 1-fructofuranosylnystose were the major oligosaccharides detected by HPLC-RID. Thus, transfructosylation activity is the main contributor to the positive correlation of 1-kestose.
fructooligosaccharides, fructosyltransferase, intracellular enzymes, chemometric characterization, extracellular enzymes
Structure type: oligomer
Location inside paper: Table 4, nystose
Trivial name: nystose, inulin, 1,1-kestotetraose
Compound class: fructan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_923067,IEDB_983931,SB_192
Methods: HPLC, enzymatic synthesis, cell growth, enzymatic assay, spectrophotometry, centrifugation, filtration
Enzymes that release or process the structure: fructosyltransferase (FTase), β-fructofuranosidase (FFase)
Synthetic data: biosynthesis
Related record ID(s): 5421, 43613, 44207, 44307, 44493, 49506, 49522, 49526, 49546, 49566, 49572, 49575, 50321, 50323, 50326, 50330, 50333, 50343, 50346, 50349, 50352, 50356, 50359, 50362, 100071, 147006, 244675, 269959
NCBI Taxonomy refs (TaxIDs): 105351,
40993,
5059,
34381,
5061,
40380,
5062,
33178Reference(s) to other database(s): GTC:G60726JL, GlycomeDB:
63, CCSD:
23519, CBank-STR:9595, GenDB:MH445969, US-PT:US2017298332A1
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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