Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
NCBI PubMed ID: 31845198Publication DOI: 10.1007/s12010-019-03215-7Journal NLM ID: 8208561Publisher: Humana Press
Correspondence: Samanta AK <drashiskumarsamanta
gmail.com>; Samanta AK <draksamanta
sac.org.bd>
Institutions: ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, India, Department of Microbiology, School of Sciences, Jain University, Bengaluru, India, SAARC Agriculture Centre, BARC Complex, Dhaka, Bangladesh
Short chain fructo-oligosaccharides (SC-FOS) are the potential prebiotics possessing diverse applications in both food and feed industries. The present study was aimed to extract inulin from chicory roots followed by its conversion into SC-FOS applying endoinulinase from Aspergillus fumigatus. The inulin was extracted from chicory roots through boiling in hot water, followed by precipitation with ethanol at room temperature or freezing condition. Maximum yield (42%) of inulin was obtained with three volumes of chilled absolute ethanol at room temperature. HPLC analysis of enzymatic hydrolysate detected kestose (GF2), nystose (GF3), and other FOS having higher degree of polymerization (DP). Maximum GF2 (5.79 mg/ml) was detected at temperature 50 °C, pH 5.5 with 2 U of enzyme dose after 6 h of hydrolysis; while maximum GF3 (4.33 mg/ml) was recorded at 60 °C, 5.5 pH with 0.5 U enzyme dose after 2 h of hydrolysis. Nevertheless, complete hydrolysis of inulin was noticed with 99% total oligosaccharide yield at 55 °C, 5.5 pH with 0.5 U enzyme dose after 4 h of hydrolysis with negligible amount of mono- and di-saccharides. The present finding demonstrated the process for higher yield of inulin from chicory roots followed by its conversion into SC-FOS applying fungal endoinulinase.
inulin, fungi, prebiotic, endoinulinase, short chain fructo-oligosaccharides
Structure type: oligomer
Location inside paper: Fig. 3, Fig. 4, Fig. 5, 1-kestose
Trivial name: 1-kestose, inulin
Compound class: fructan, fructo-oligosaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
Methods: IR, TLC, HPLC, enzymatic digestion, extraction, cell growth, enzymatic assay, precipitation, phenol-sulfuric acid assay, centrifugation, filtration, Somogyi-Nelson method
Enzymes that release or process the structure: endoinulinase
Synthetic data: enzymatic
Related record ID(s): 5201, 43612, 44206, 44306, 44492, 45817, 49006, 49009, 49505, 49521, 49525, 49545, 49557, 49565, 49569, 49571, 49574, 50321, 50326, 50329, 50332, 50342, 50345, 50348, 50351, 50355, 50358, 50361, 147005, 213017, 219959, 245753, 246867
NCBI Taxonomy refs (TaxIDs): 746128Reference(s) to other database(s): GTC:G11260CN, GlycomeDB:
396, CCSD:
26122, CBank-STR:5624, GenDB:MH445969, US-PT:US2017298332A1
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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