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Capsular polysaccharides were isolated from A. baumannii LUH5535 (K35 CPS) and LUH5554 (K15 CPS) and studied by 1D and 2D 1H and 13C NMR spectroscopy. The CPSs were found to consist of linear tetrasaccharide repeats (K units) containing 2-acetamido-2-deoxy-d-galacturonic acid (K35) or 2,3-diacetamido-2,3-deoxy-d-glucuronic acid (K15) and 2,4-diacetamido-2,4,6-trideoxy-d-glucose (both CPSs). The K35 unit includes three O-acetyl groups on different GalNAcA residues. A. baumannii LUH5535 and LUH5554 carry the KL35 and KL15 gene clusters, respectively, and putatively assigned functions of genes in these clusters were consistent with the CPS structures established.

Acinetobacter baumannii, capsular polysaccharide structure, 2, 4-diacetamido-2, 4, 6-trideoxy-D-glucose, 2-acetamido-2-deoxy-D-galacturonic acid, 3-diacetamido-2, Enterobacter cloacae, K locus, 3-deoxy-d-glucuronic acid

NCBI PubMed ID: 28578199
Publication DOI: 10.1016/j.carres.2017.05.017
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: yknirel

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Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, mild alkaline degradation, GPC, bioinformatic analysis