Haseley SR, Wilkinson SG Structure of the O-specific polysaccharide of Acinetobacter baumannii O5 containing 2-acetamido-2-deoxy-D-galacturonic acid European Journal of Biochemistry237 (1996)
229-233
The structure was elucidated in this paper NCBI PubMed ID:8620877 Publication DOI:10.1111/j.1432-1033.1996.0229n.x Journal NLM ID:0107600 Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Institutions: School of Chemistry, University of Hull, England.
polysaccharide containing 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamido-2-deoxy-L-fucose (FucNAc), and 2-acetamido-2-deoxy-D-galacturonic acid (GalNAcA) was isolated from an aqueous phenol extract of lipid-free, isolated cell walls of the reference strain for Acinetobacter baumannii serogroup O5, by mild acid hydrolysis of the extract and chromatography of the water-soluble products on Sephadex G-50. By means of NMR studies, methylation analysis, carboxyl reduction and chemical degradations, the repeating unit of the polymer was identified as a branched tetrasaccharide of the structure shown. The serologically active polymer is believed to correspond to the side chain of the O5 lipopolysaccharide: [table: see text]
Methods: methylation, NMR-2D, partial acid hydrolysis, NMR, carboxyl reduction, Smith degradation Comments, role: the disaccharide is product of partial hydrolysis of the carboxyl-reduced polymer
Related record ID(s): 487 NCBI Taxonomy refs (TaxIDs):470 Reference(s) to other database(s): GTC:G79056JA, GlycomeDB:8234 Show glycosyltransferases
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