Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32429204Publication DOI: 10.3390/vaccines8020226Journal NLM ID: 101629355Publisher: Basel, Switzerland: MDPI AG
Correspondence: Kjærup RB <rikke.kjaerup
anis.au.dk>; Guldbrandtsen B <bernt.guldbrandtsen
mbg.au.dk>; Christensen D <den
ssi.dk>; Pitcovski J <jp
migal.org.il>; Larsen FT <ftl
anis.au.dk>; Dalgaard TS <tina.dalgaard
anis.au.dk>
Institutions: Department of Animal Science, Aarhus University, Tjele, Denmark, Center for Quantitative Genetics and Genomics, Tjele, Denmark, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark, Virology and Vaccine Development Laboratory, MIGAL Technology Center, Kiryat Shmona, Israel
Infectious bronchitis virus (IBV) is a highly contagious avian coronavirus. IBV causes substantial worldwide economic losses in the poultry industry. Vaccination with live-attenuated viral vaccines, therefore, are of critical importance. Live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. Therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subunit vaccines are less immunogenic than live-attenuated vaccines. Several glycan-based adjuvants directly targeting mammalian C-type lectin receptors were assessed in vitro using chicken bone marrow-derived dendritic cells (BM-DCs). The β-1-6-glucan, pustulan, induced an up-regulation of MHC class II (MHCII) cell surface expression, potentiated a strong proinflammatory cytokine response, and increased endocytosis in a cation-dependent manner. Ex vivo co-culture of peripheral blood monocytes from IBV-immunised chickens, and BM-DCs pulsed with pustulan-adjuvanted recombinant IBV N protein (rN), induced a strong recall response. Pustulan-adjuvanted rN induced a significantly higher CD4+ blast percentage compared to either rN, pustulan or media. However, the CD8+ and TCRγδ+ blast percentage were significantly lower with pustulan-adjuvanted rN compared to pustulan or media. Thus, pustulan enhanced the efficacy of MHCII antigen presentation, but apparently not the cross-presentation on MHCI. In conclusion, we found an immunopotentiating effect of pustulan in vitro using chicken BM-DCs. Thus, future in vivo studies might show pustulan as a promising glycan-based adjuvant for use in the poultry industry to contain the spread of coronaviridiae as well as of other avian viral pathogens.
adjuvant, chicken, Pustulan, APC-targeting, BM-DC, IBV, subunit vaccination
Structure type: homopolymer
Location inside paper: abstract
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: PCR, biological assays, cell growth, flow cytometry analysis, confocal microscopy, DNA extraction, centrifugation, gel electrophoresis, qRT-PCR, endocytosis assay
Biological activity: pustulan induced an up-regulation of MHC class II (MHCII) cell surface expression, potentiated a strong proinflammatory cytokine response, and increased endocytosis in a cation-dependent manner
NCBI Taxonomy refs (TaxIDs): 136370Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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