Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Publication DOI: 10.1016/j.bcab.2019.101455Journal NLM ID: 101589270Publisher: Atlanta, GA: Elsevier
Correspondence: Wan-Mohtar WAAQI <qadyr
um.edu.my>
Institutions: Agro-Biotechnology Institute, Malaysia (ABI), National Institutes of Biotechnology Malaysia (NIMB), Serdang, Malaysia, Mushroom Research Centre and Functional Foods and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia, Biomass Energy Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia, Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur, Malaysia, Halal Action Laboratory, Pusat Genius Insan, University Sains Islam Malaysia, Nilai, Malaysia, Bioresources and Bioprocessing Research Group, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
A rare Malaysian tiger milk mushroom Lignosus rhinocerus strain ABI (LRSA) was morphologically identified based on its pileus, stipe, and sclerotium. LRSA (515 bp) was sequenced and found to have 99% similar to L. rhinocerus strains CH31 and CH2. Phylogenetically, evolutionary distance (Knuc) and plasmid-matching software (ApE) for sequences of matching fungal species were used to verify that the isolate belonged to the L. rhinocerus species. The strain was cultured in a stirred-tank bioreactor and a mycelial β-glucan (G) was extracted for compound characterization. The structure of exopolysaccharide extract from mycelium of LRSA was studied using Fourier-transform infrared spectroscopy (FT-IR) and one-dimensional (1D) and two-dimensional (2D) Nuclear Magnetic Resonance (NMR). FT-IR spectroscopy showed that G exhibited a similar β-glycosidic structure to the standard (laminarin), and the presence of characteristic bands at 3277, 2919, 1638, 1545, 1400, 1078, and 896 1/cm confirmed the similarities. 1H and 13C NMR, as well as 2D NMR: homonuclear correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), heteronuclear multiple quantum coherence (HMQC), and heteronuclear multiple bond coherence (HMBC) spectra, were used for structural elucidation of the β-glucan and confirmed the extracted material as (1,3)-β-D-glucan. In addition, the G compound exhibited antioxidant activities through total phenolic content (4.47 mg gallic acid equivalents/g), 2, 2-diphenyl-1-picrylhydrazyl (11.48 mg/mL), and ferric reducing antioxidant power (0.56 mg/mL) assays. These findings may facilitate the development of rare G production in a high-scale bioreactor using LRSA.
2D NMR, Antioxidant, (1, 3)-β-D-glucan, Lignosus rhinocerus, tiger milk mushroom, bioreactor fermentation
Structure type: homopolymer
Location inside paper: Table 1
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, IR, PCR, enzymatic digestion, extraction, cell growth, enzymatic assay, antioxidant activities, DNA extraction, precipitation, spectrophotometry, centrifugation, filtration, BLAST, Folin phenol reagent method
Biological activity: polysaccharide has effective antioxidant properties with high free radical scavenging activity
Comments, role: published suspicious NMR spectrum of -3)bDGlcp(1- (13C: #_bDGlcp 4.60 3.23 3.47 3.92 3.37 3.69-3.89 //; 1H: #_bDGlcp 4.60 3.23 3.47 3.92 3.37 3.69-3.89 //) was removed by CSDB staff
NCBI Taxonomy refs (TaxIDs): 483020Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
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There is only one chemically distinct structure:
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Found 1 record.
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(later renamed to: Lignosus rhinocerotis ABI)