Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 31826486Publication DOI: 10.1016/j.carbpol.2019.115521Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Morales D <diego.morales
uam.es>; Rutckeviski R <renatarut
hotmail.com>; Villalva M, <marisol.villalva
uam.es>; Abreu H <ha.hellenabreu
gmail.com>; Soler-Rivas C <cristina.soler
uam.es>; Santoyo S <susana.santoyo
uam.es>; Iacomini M <iacomini
ufpr.br>; Smiderle FR <fhernandas
gmail.com>
Institutions: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Faculdades Pequeno Príncipe, Curitiba, Brazil, Department of Production and Characterization of Novel Foods, Institute of Food Science Research - CIAL (UAM+CSIC), Campus de Cantoblanco, Universidad Autónoma de Madrid, Madrid, Spain, Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Brazil
A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with β-(1→6), β-(1→3),(1→6) and α-(1→3) linkages, being characterized through GC-MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory and antioxidant activities were also tested. Isolated glucans exerted HMGCR inhibitory activity, but only β-(1→6) and β-(1→3),(1→6) fractions showed DPPH scavenging capacity. Glucans were also able to lower IL-1β and IL-6 secretion by LPS-activated THP-1/M cells and showed cytotoxic effect on a breast cancer cell line that was not observed on normal breast cells. These in vitro results pointed important directions for further in vivo studies, showing different effects of each chemical structure of the isolated glucans from shiitake mushrooms.
β-Glucans, α-glucans, anti-inflammatory, cytotoxic, shiitake mushroom, hypocholesterolemic
Structure type: homopolymer
Location inside paper: Fig. 3, a
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, IR, GC-MS, inhibition studies, acid hydrolysis, extraction, acetylation, fluorescence spectroscopy, SEC, reduction, dialysis, antioxidant activities, cytotoxicity assay, precipitation, antitumor activity assay, Congo Red assay, centrifugation, MTT
Related record ID(s): 50872, 50873
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 343 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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