Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 34973736Publication DOI: 10.1016/j.carbpol.2021.118917Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: F.R. Smiderle <fhernanda.smiderle
professor.fpp.edu.br>
Institutions: Faculdades Pequeno Príncipe, 80230-020 Curitiba, PR, Brazil, Instituto de Pesquisa Pelé Pequeno Príncipe, 80240-020 Curitiba, PR, Brazil, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR 81531-980, Brazil
Mushroom β-D-glucans have demonstrated immunomodulatory activity, which is initiated by their recognition by specific receptors on immune system cells surfaces. Studies indicated that β-D-glucans may present a synergistic effect with chemotherapy drugs. In this study, a linear β-(1→6)-D-glucan (B16), isolated from A. bisporus and previously characterized (Mw: 8.26 × 104 g/mol), was evaluated about its capacity to modulate THP-1 macrophages towards an M1 phenotype and induce an antitumoral activity. This was evidenced by the production of pro-inflammatory markers upon B16 treatment (30; 100 μg/mL). The breast tumor cells (MDA-MB-231) viability was not affected by treatment with B16, however, their viability markedly decreased upon treatment with the drug doxorubicin. The results showed a synergic effect of B16 and doxorubicin, which reduced the viability of MDA-MB-231 cells by 31%. Furthermore, B16 treatment provided a sustainable M1 state environment and contributed to increase the sensitivity of breast cancer cells to the doxorubicin treatment.
immunomodulatory activity, Antioxidant activity, Agaricus bisporus, antitumoral effect, β-(1→6)-D-glucan, triple-negative breast cancer cells
Structure type: homopolymer ; 82600
Trivial name: β-(1→6)-D-glucan (B16)
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: cytokine analysis, statistical analysis, flow cytometry analysis, determination of NO production, cell viability assay, radical scavenging assay, protein determination, immunomodulatory activity analysis, oxidative stress
NCBI Taxonomy refs (TaxIDs): 5341Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
There is only one chemically distinct structure:
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